Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein

dc.creatorMartins, D
dc.creatorFrungillo, L
dc.creatorAnazzetti, MC
dc.creatorMelo, PS
dc.creatorDuran, N
dc.date2010
dc.date2014-11-16T04:16:23Z
dc.date2015-11-26T17:23:18Z
dc.date2014-11-16T04:16:23Z
dc.date2015-11-26T17:23:18Z
dc.date.accessioned2018-03-29T00:10:39Z
dc.date.available2018-03-29T00:10:39Z
dc.identifierInternational Journal Of Nanomedicine. Dove Medical Press Ltd, v. 5, n. 77, n. 85, 2010.
dc.identifier1176-9114
dc.identifierWOS:000283715300007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54697
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54697
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54697
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283799
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionIt has been demonstrated that tumoral cells have a higher uptake of ascorbic acid compared to normal cells. This differential characteristic can be used as a way to improve the specificity of antitumoral compounds if combined with polymeric drug delivery systems. The aim of this study was to prepare, characterize and evaluate the antitumoral activity of poly-D,L-(lactide-co-glycolide) 50: 50 loading the antitumoral compound violacein and capped with L-ascorbic acid. Nanoparticles were prepared using the nanoprecipitation method and morphologically characterized by scanning electron microscopy (SEM). The average diameter and Zeta potential were determined by photon correlation spectroscopy method (PCS), and assays were carried out to determine the content of ascorbic acid and in vitro drug release kinetics. The antitumoral activity of this system was also evaluated against HL-60 cells by tetrazolium reduction assay. Nanoparticles with size distribution between 300-400 nm and strong negative outer surface (-40 mV) were obtained by this method. Analysis of ascorbic acid content showed that this compound was mainly localized on the external surface of nanoparticles. Violacein loading efficiency was determined as 32% +/- 1% and this drug was gradually released from nanoparticles at different rates depending on the composition of the release media. In addition, this system was observed to be 2 x more efficient as an antitumoral compared with free violacein.
dc.description5
dc.description77
dc.description85
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionBrazilian Nanobiotechnology Network
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherDove Medical Press Ltd
dc.publisherAlbany
dc.publisherNova Zelândia
dc.relationInternational Journal Of Nanomedicine
dc.relationInt. J. Nanomed.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectviolacein
dc.subjectascorbic acid
dc.subjectnanoparticles
dc.subjectPLGA
dc.subjectCancer-therapy
dc.subjectChromobacterium-violaceum
dc.subjectVitamin-c
dc.subjectGlucose Transporters
dc.subjectCell-lines
dc.subjectIn-vivo
dc.subjectDelivery
dc.subjectTherapeutics
dc.subjectNanocarriers
dc.subjectInhibition
dc.titleAntitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
dc.typeArtículos de revistas


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