Decreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expression

dc.creatorFerreira, F
dc.creatorBarbosa, HCL
dc.creatorStoppiglia, LF
dc.creatorDelghingaro-Augusto, V
dc.creatorPereira, EA
dc.creatorBoschero, AC
dc.creatorCarneiro, EM
dc.date2004
dc.dateJAN
dc.date2014-11-15T22:54:30Z
dc.date2015-11-26T17:22:25Z
dc.date2014-11-15T22:54:30Z
dc.date2015-11-26T17:22:25Z
dc.date.accessioned2018-03-29T00:09:52Z
dc.date.available2018-03-29T00:09:52Z
dc.identifierJournal Of Nutrition. Amer Inst Nutrition, v. 134, n. 1, n. 63, n. 67, 2004.
dc.identifier0022-3166
dc.identifierWOS:000188124900011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79351
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79351
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79351
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283600
dc.descriptionA low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 mumol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the alpha catalytic subunit of protein kinase A (PKAalpha) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAalpha mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAalpha expression. These alterations in PKAalpha may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet.
dc.description134
dc.description1
dc.description63
dc.description67
dc.languageen
dc.publisherAmer Inst Nutrition
dc.publisherBethesda
dc.publisherEUA
dc.relationJournal Of Nutrition
dc.relationJ. Nutr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectlow protein diet
dc.subjectforskolin
dc.subjectinsulin secretion
dc.subjectprotein kinase alpha
dc.subjectgene expression
dc.subjectPancreatic Beta-cells
dc.subjectKinase-c Activation
dc.subjectCalorie Malnutrition
dc.subjectEnergy Malnutrition
dc.subjectRegulatory Subunits
dc.subjectFood Restriction
dc.subjectGene-expression
dc.subjectLimited Period
dc.subjectSertoli Cells
dc.subjectAdult-rats
dc.titleDecreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expression
dc.typeArtículos de revistas


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