Role of F1F0-ATP synthase and uncoupling protein in mitochondrial respiratory uncoupling of ripened avocado fruits

dc.creatorSaviani, EE
dc.creatorda Silva, A
dc.creatorMartins, IS
dc.date1998
dc.dateAUG
dc.date2014-12-02T16:25:25Z
dc.date2015-11-26T17:22:19Z
dc.date2014-12-02T16:25:25Z
dc.date2015-11-26T17:22:19Z
dc.date.accessioned2018-03-29T00:09:47Z
dc.date.available2018-03-29T00:09:47Z
dc.identifierPlant Physiology And Biochemistry. Gauthier-villars/editions Elsevier, v. 36, n. 8, n. 575, n. 582, 1998.
dc.identifier0981-9428
dc.identifierWOS:000075710700005
dc.identifier10.1016/S0981-9428(98)80005-3
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71216
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71216
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71216
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283579
dc.descriptionThe present study was carried out to determine the contribution of the F-1 F-0-ATP synthase, the ATP/ADP carrier (AAC) and a putative plant uncoupling mitochondrial protein (PUMP) to the increased respiratory activity of ripened avocado fruits. We have compared the beta-subunit content of F-1 and AAC, determined by photoaffinity labelling with 8-azido-[alpha-P-32]ATP, and the transmembrane electrical potential difference (Delta Psi), established by substrate oxidation, in mitochondria isolated from pre- and postclimacteric avocado fruits. The beta-subunit content of F-1 decreased, while that of AAC did not change, with ripening. The Delta Psi established by succinate oxidation of postclimacteric avocado mitochondria was very low and could be substantially increased by oligomycin, which inhibits H+ transport through F-0, and by the purine nucleotides GTP or ATP, which are known to inhibit H+ transport through the uncoupling protein present in brown adipose tissue and potato tuber mitochondria. Oligomycin and GTP had no effect on the Delta Psi of preclimacteric avocado mitochondria. Carboxyatractyloside and bongkrekic acid, specific inhibitors of AAC, had no effect on the Delta Psi of pre- or postclimacteric avocado mitochondria. These results suggest that a PUMP-like protein may exist in mitochondria of postclimacteric avocado fruits, and that this protein and the F-0 from beta-detached ATP synthase, but not the AAC, could provide pathways for energy dissipation in ripened avocado fruit mitochondria. (C) Elsevier, Paris.
dc.description36
dc.description8
dc.description575
dc.description582
dc.languageen
dc.publisherGauthier-villars/editions Elsevier
dc.publisherParis
dc.publisherFrança
dc.relationPlant Physiology And Biochemistry
dc.relationPlant Physiol. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectavocado fruit ripening
dc.subjectalternative oxidase
dc.subjectmitochondrial ATP synthase
dc.subjectplant uncoupling mitochondrial protein
dc.subjectphotoaffinity labelling
dc.subjectATP/ADP carrier
dc.subjectmitochondrial H+ permeability
dc.subjectBrown-adipose-tissue
dc.subjectFatty-acids
dc.subjectAlternative Oxidase
dc.subjectOxidative-phosphorylation
dc.subjectProton Conductance
dc.subjectPlant-mitochondria
dc.subjectH+ Transport
dc.subjectMechanism
dc.subjectNucleotides
dc.subjectMembrane
dc.titleRole of F1F0-ATP synthase and uncoupling protein in mitochondrial respiratory uncoupling of ripened avocado fruits
dc.typeArtículos de revistas


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