Dehydrocrotonin and its beta-cyclodextrin complex: Cytotoxicity in V79 fibroblasts and rat cultured hepatocytes

dc.creatorCorrea, DHA
dc.creatorMelo, PS
dc.creatorde Carvalho, CAA
dc.creatorde Azevedo, MBM
dc.creatorDuran, N
dc.creatorHaun, M
dc.date2005
dc.date39142
dc.date2014-11-15T22:32:53Z
dc.date2015-11-26T17:22:16Z
dc.date2014-11-15T22:32:53Z
dc.date2015-11-26T17:22:16Z
dc.date.accessioned2018-03-29T00:09:44Z
dc.date.available2018-03-29T00:09:44Z
dc.identifierEuropean Journal Of Pharmacology. Elsevier Science Bv, v. 510, n. 41671, n. 17, n. 24, 2005.
dc.identifier0014-2999
dc.identifierWOS:000227633300003
dc.identifier10.1016/j.ejphar.2005.01.016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79412
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79412
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79412
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283565
dc.descriptionTrans-dehydrocrotonin has antiulcerogenic and antitumor activities. A complex of beta-cyclodextrin with dehydrocrotonin was developed to improve the delivery of dehydrocrotonin. Complex in solid state was evaluated using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). X-ray diffraction and scanning electron microscopy studies showed that dehydrocrotonin exists in a semicrystalline state in the complexed form with beta-cyclodextrin. Differential scanning calorimetry studies showed the existence of a complex of dehydrocrotonin with beta-cyclodextrin. The thermal gravimetric analysis studies confirmed the differential scanning calorimetry results of the complex. Free dehydrocrotonin and the dehydrocrotonin/ beta-cyclodextrin inclusion complex were assayed in freshly isolated rat hepatocytes and in V79 cells. Cytotoxicity was determined using nucleic acid content, methylthiazoletetrazolium (MTT) reduction and neutral red uptake assays. In all assays, there was a large reduction (3.5-16.1-fold) in the cytotoxicity of dehydrocrotonin in hepatocytes when complexed with p-cyclodextrin, whereas for V79 cells the decrease in cytotoxicity was 1.7- and 1.87-fold for MTT reduction and nucleic acid content assays, respectively. The lower cytotoxicity of the dehydrocrotonin/beta cyclodextrin complex compared to free dehydrocrotonin in rat hepatocytes and V79 cells suggests that such a complex may be useful for the administration of dehydrocrotonin in vivo. (c) 2005 Elsevier B.V. All rights reserved.
dc.description510
dc.description41671
dc.description17
dc.description24
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationEuropean Journal Of Pharmacology
dc.relationEur. J. Pharmacol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectbeta-cyclodextrin
dc.subjectcytotoxicity
dc.subjectdehydrocrotonin
dc.subjecthepatocyte
dc.subjectV79 cell
dc.subjectCroton-cajucara
dc.subjectTrans-dehydrocrotonin
dc.subjectInclusion Complex
dc.subjectDrug-delivery
dc.subjectDiterpene
dc.subjectDerivatives
dc.subjectMechanisms
dc.subjectChemistry
dc.subjectRelease
dc.subjectSystems
dc.titleDehydrocrotonin and its beta-cyclodextrin complex: Cytotoxicity in V79 fibroblasts and rat cultured hepatocytes
dc.typeArtículos de revistas


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