Chromatin supraorganization and extensibility in mouse hepatocytes with development and aging

dc.creatorMoraes, AS
dc.creatorGuaraldo, AMA
dc.creatorMello, MLS
dc.date2007
dc.dateJAN
dc.date2014-11-15T18:57:33Z
dc.date2015-11-26T17:21:24Z
dc.date2014-11-15T18:57:33Z
dc.date2015-11-26T17:21:24Z
dc.date.accessioned2018-03-29T00:08:55Z
dc.date.available2018-03-29T00:08:55Z
dc.identifierCytometry Part A. Wiley-liss, v. 71A, n. 1, n. 28, n. 37, 2007.
dc.identifier1552-4922
dc.identifierWOS:000243900300006
dc.identifier10.1002/cyto.a.20356
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55843
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55843
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55843
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283357
dc.descriptionBackground: Chromatin supraorganization and extensibility, which lead to the formation of extended chromatin fibers (ECF), are affected by starvation and refeeding in adult mouse hepatocytes. It is expected that they could also change with mouse development and aging. Methods: Methods used involved topochemistry image analysis, microspectrophotometry, gravity action, and polarization microscopy Results: Increased nuclear areas and Feulgen-DNA amounts with advancing hepatocyte polyploidy were found with development and aging. A slightly less packed chromatin with more heterogeneously distributed condensation levels was detected in young and old mice. Con-A responsiveness was almost absent in young mice but very deep in aged mice. ECFs formed from nuclei of adult and aged mice but not from nuclei of young mice. The frequency of ECF formation with the long lysis protocol increased with aging. Conclusions: in young mice, a less packed chromatin state may be associated with more intense gene activity, thus increasing the DNA-nuclear matrix interactions, and inhibiting ECF formation. Reduced DNA-nuclear matrix interactions besides defects in heterochromatin formation may induce higher ECF formation and chromatin unpackaging in old mice. We suggest that differences in Con-A staining relate to different gene activity with advancing development and aging. (c) 2007 international Society for Analytical Cytology
dc.description71A
dc.description1
dc.description28
dc.description37
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationCytometry Part A
dc.relationCytom. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectchromatin supraorganization
dc.subjectextended chromatin fibers
dc.subjectdevelopment
dc.subjectaging
dc.subjectCon-A-positive glycoproteins
dc.subjectpolarization microscopy
dc.subjectmicrospectrophotometry
dc.subjectIn-situ Hybridization
dc.subjectExtended Chromatin
dc.subjectNuclear-matrix
dc.subjectConcanavalin-a
dc.subjectHistone Methylation
dc.subjectSatellite Dna
dc.subjectO-glcnac
dc.subjectHeterochromatin
dc.subjectProteins
dc.subjectLiver
dc.titleChromatin supraorganization and extensibility in mouse hepatocytes with development and aging
dc.typeArtículos de revistas


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