Cholesteryl ester transfer protein expression is down-regulated in hyperinsulinemic transgenic mice

dc.creatorBerti, JA
dc.creatorCasquero, AC
dc.creatorPatricio, PR
dc.creatorBighetti, EJB
dc.creatorCarneiro, EM
dc.creatorBoschero, AC
dc.creatorOliveira, HCF
dc.date2003
dc.dateOCT
dc.date2014-11-15T18:37:17Z
dc.date2015-11-26T17:21:18Z
dc.date2014-11-15T18:37:17Z
dc.date2015-11-26T17:21:18Z
dc.date.accessioned2018-03-29T00:08:49Z
dc.date.available2018-03-29T00:08:49Z
dc.identifierJournal Of Lipid Research. Lipid Research Inc, v. 44, n. 10, n. 1870, n. 1876, 2003.
dc.identifier0022-2275
dc.identifierWOS:000186043900007
dc.identifier10.1194/jlr.M300036-JLR200
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55837
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55837
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55837
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283335
dc.descriptionCholesteryl ester transfer protein (CETP) mediates cholesteryl ester (CE) and triglyceride redistribution among plasma lipoproteins. In this work, we investigated whether varying levels of insulin regulate the CETP expression in vivo. Insulin deficiency [streptozotocin (STZ) injection], and hyperinsulinemia (insulin injections, 14 days) were induced in transgenic mice expressing a human CETP minigene flanked by its natural regulatory sequences. Glucose supplementation was provided to the hyperinsulinemic group (INS+GLUC) and to an extra group of mice (GLUC). In the STZ group, endogenous CE transfer rate, plasma CETP, and hepatic CETP mRNA levels were enhanced 3.0-, 1.5-, and 2.5-fold, respectively, as compared with controls. Insulin replacement in STZ mice normalized their glycemia and liver mRNA levels. Higher plasma CETP levels were observed in GLUC mice, which were decreased in INS+GLUC mice. Hepatic CETP mRNA was not altered in GLUC mice and was reduced by one-third in INS+GLUC mice. jlr These results show that: 1) STZ treatment increases CETP plasma levels and liver mRNA expression; 2) diet glucose supplementation increases plasma CETP levels but does not change liver mRNA abundance; and 3) daily insulin injections blunt the glucose-stimulated CETP expression by reducing its liver mRNA levels. These data suggest that insulin down-regulates CETP gene expression.-Berti, J. A., A. C. Casquero, P. R. Patricio, E. J. B. Bighetti, E. M. Carneiro, A. C. Boschero, and H. C. E Oliveira. Cholesteryl ester transfer protein expression is down-regulated in hyperinsulinemic transgenic mice. J. Lipid Res. 2003. 44: 1870-1876.
dc.description44
dc.description10
dc.description1870
dc.description1876
dc.languageen
dc.publisherLipid Research Inc
dc.publisherBethesda
dc.publisherEUA
dc.relationJournal Of Lipid Research
dc.relationJ. Lipid Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcholesteryl ester transfer protein gene expression
dc.subjectinsulin
dc.subjecthyperglycemia
dc.subjectexperimental diabetes
dc.subjectlipoprotein lipase
dc.subjecttriglycerides
dc.subjectnonesterified fatty acids
dc.subjectElement-binding Protein-1c
dc.subjectHigh-density-lipoproteins
dc.subjectLipid Transfer Protein
dc.subjectSterol Up-regulation
dc.subjectGene-expression
dc.subjectAdipose-tissue
dc.subjectDiabetes-mellitus
dc.subjectInsulin Action
dc.subjectMessenger-rna
dc.subjectIn-vitro
dc.titleCholesteryl ester transfer protein expression is down-regulated in hyperinsulinemic transgenic mice
dc.typeArtículos de revistas


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