Evidence for the involvement of a macrophage-derived chemotactic mediator in the neutrophil recruitment induced by staphylococcal enterotoxin B in mice

dc.creatorDesouza, IA
dc.creatorHyslop, S
dc.creatorFranco-Penteado, CF
dc.creatorRibeiro-DaSilva, G
dc.date2002
dc.dateDEC
dc.date2014-11-17T09:20:04Z
dc.date2015-11-26T17:21:01Z
dc.date2014-11-17T09:20:04Z
dc.date2015-11-26T17:21:01Z
dc.date.accessioned2018-03-29T00:08:35Z
dc.date.available2018-03-29T00:08:35Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 40, n. 12, n. 1709, n. 1717, 2002.
dc.identifier0041-0101
dc.identifierWOS:000180152300009
dc.identifier10.1016/S0041-0101(02)00237-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66088
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66088
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66088
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283272
dc.descriptionStaphylococcus aureus secretes enterotoxins which are superantigens and the major cause of food poisoning in man. Staphylococcal enterotoxins types A and B can induce neutrophil migration into the peritoneal cavity of mice through sensory C-fiber neuropeptides, lipoxygenase or cyclooxygenase metabolites, nitric oxide, histamine, platelet-activating factor and resident macrophages. In this work, we examined the influence of macrophage-derived products on neutrophil migration during peritonitis induced by staphylococcal enterotoxin type B (SEB) in mice. Macrophages stimulated with SEB released a thermolabile neutrophil chemotactic protein with a molecular weight of 1000-3000 (by ultrafiltration). This release was inhibited 30% by dexamethasone (an inhibitor of cytokine synthesis and phospholipase A(2) activity), but not by indomethacin (a cyclooxygenase inhibitor) or BW755C (a dual cyclo- and lipoxygenase inhibitor). Dexamethasone also inhibited (100%) the neutrophil migration induced by the chemotactic protein. Similar inhibition occurred in mice pretreated with BWA4C (lipoxygenase inhibitor; 90%), BW755C (99%), BN52021 (platelet-activating factor-acether receptor antagonist; 93%), cimetidine (histamine H-2 receptor antagonist; 76%), capsaicin (a depletor of sensory C-fiber neuropeptides; 82%) and the neurokinin-1 receptor antagonist SR140333 (71%), but not by indomethacin or the neurokinin(2) receptor antagonist SR48968. These results confirm that macrophages are involved in the neutrophil recruitment induced by SEB, and that the chemotactic protein apparently induces neutrophil migration by a mechanism mediated by platelet-activating factor, histamine H2 receptors, lipoxygenase products and substance P. (C) 2002 Elsevier Science Ltd. All rights reserved.
dc.description40
dc.description12
dc.description1709
dc.description1717
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectinterleukins
dc.subjectmacrophages
dc.subjectneurogenic inflammation
dc.subjectneutrophil migration
dc.subjectperitoneal cavity
dc.subjectstaphylococcal enterotoxin type B
dc.subjectMouse Paw Edema
dc.subjectGuinea-pig Skin
dc.subjectSubstance-p
dc.subjectVascular-permeability
dc.subjectUnsensitized Monkeys
dc.subjectResident Macrophages
dc.subjectMigration
dc.subjectInflammation
dc.subjectInvivo
dc.subjectInhibition
dc.titleEvidence for the involvement of a macrophage-derived chemotactic mediator in the neutrophil recruitment induced by staphylococcal enterotoxin B in mice
dc.typeArtículos de revistas


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