Toluene gas phase biofiltration by Paecilomyces lilacinus and isolation and identification of a hydrophobin protein produced thereof

dc.creatorVigueras, G
dc.creatorShirai, K
dc.creatorMartins, D
dc.creatorFranco, TT
dc.creatorFleuri, LF
dc.creatorRevah, S
dc.date2008
dc.dateAUG
dc.date2014-11-15T15:20:20Z
dc.date2015-11-26T17:20:36Z
dc.date2014-11-15T15:20:20Z
dc.date2015-11-26T17:20:36Z
dc.date.accessioned2018-03-29T00:08:12Z
dc.date.available2018-03-29T00:08:12Z
dc.identifierApplied Microbiology And Biotechnology. Springer, v. 80, n. 1, n. 147, n. 154, 2008.
dc.identifier0175-7598
dc.identifierWOS:000257485900016
dc.identifier10.1007/s00253-008-1490-6
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77697
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77697
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77697
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283175
dc.descriptionPaecilomyces lilacinus consumed toluene as the sole carbon source in a gas-phase biofilter packed with perlite obtaining an average elimination capacity of 50 g m(-3) h(-1), a removal efficiency of 53%, and a final biomass of 31.6 mg biomass g dry support(-1). Hydrophobin proteins from the mycelium produced in the biofilter were purified by formic acid extraction and precipitated by electrobubbling, and the molecular weight was found to be 10.6 +/- 0.3 kDa. The peptide mass fingerprinting analysis of the purified hydrophobin by matrix-assisted laser desorption/ionization time-of-flight resulted in the identification of two peptides that presented high homology with sequences of class I hydrophobin proteins from other ascomycetous fungi when compared against the National Center for Biotechnology Information database. The yield of hydrophobin (PLHYD) from P. lilacinus was 1.1 mg PLHYD g biomass(-1). These proteins modified the hydrophobicity of Teflon by lowering the contact angle from 130.1 (+/- 2) to 57.0 (+/- 5 supporting hot sodium dodecyl sulfate washing. This work is the first report about biodegradation of toluene by the nematophagous fungus P. lilacinus in a gas-phase biofilter and the identification of its hydrophobin protein.
dc.description80
dc.description1
dc.description147
dc.description154
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationApplied Microbiology And Biotechnology
dc.relationAppl. Microbiol. Biotechnol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectfungal biofilter
dc.subjectPaecilomyces lilacinus
dc.subjecthydrophobicity
dc.subjecthydrophobins
dc.subjectBeauveria-bassiana
dc.subjectWaste Gases
dc.subjectFungal Hydrophobins
dc.subjectFusarium-solani
dc.subjectRing-cleavage
dc.subjectRemoval
dc.subjectBiofilter
dc.subjectHexane
dc.subjectSurface
dc.subjectHydrocarbons
dc.titleToluene gas phase biofiltration by Paecilomyces lilacinus and isolation and identification of a hydrophobin protein produced thereof
dc.typeArtículos de revistas


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