The capacity of connective tissue stromas to sustain myelopoiesis depends both upon the growth factors and the local intercellular environment

dc.creatorCarvalho, MA
dc.creatorArcanjo, K
dc.creatorSilva, LCF
dc.creatorBorojevic, R
dc.date2000
dc.dateDEC
dc.date2014-12-02T16:24:52Z
dc.date2015-11-26T17:20:02Z
dc.date2014-12-02T16:24:52Z
dc.date2015-11-26T17:20:02Z
dc.date.accessioned2018-03-29T00:07:39Z
dc.date.available2018-03-29T00:07:39Z
dc.identifierBiology Of The Cell. Editions Scientifiques Medicales Elsevier, v. 92, n. 41890, n. 605, n. 614, 2000.
dc.identifier0248-4900
dc.identifierWOS:000168595800006
dc.identifier10.1016/S0248-4900(01)01113-3
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78104
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78104
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78104
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283036
dc.descriptionIn adults, haemopoiesis is located in the bone marrow, where it is tightly regulated by cytokines and by a physical association of haemopoietic progenitors with the stroma. However, in pathological situations, haemopoiesis can be partly or fully dislodged to peripheral tissues. It is not clear which are the requirements for a given peripheral stroma to sustain haemopoiesis. Using the growth factor-dependent cell line FDC-P1, we have compared the myelopoietic capacities of a murine bone marrow-derived cell line S17, a liver inflammatory granuloma-derived stroma (GR) that sustains haemopoiesis, and normal skin fibroblasts (SF) that sustain neither survival nor proliferation of myeloid cells. All three stromas expressed mRNA for major haemopoietins with the exception of IL-3. Despite the incapacity of SF to sustain FDC-P1 cells, the biologically active GM-CSF could be recovered from all the studied stromas by treatment with high-salt buffers that release non-covalently bound molecules from stroma cells. Glycosaminoglycans purified from stromas had distinct effect on the GM-CSF-mediated proliferation of FDC-P1 cells: those purified from S17 and GR cells were stimulatory, whereas those obtained from SF cells were slightly stimulatory at low concentration, but inhibitory at the higher ones. We conclude that the quality of the stroma pericellular glycoconjugates is determinant for the ability of a given stroma to sustain myelopoiesis, even when biologically active haemopoietins are locally produced. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
dc.description92
dc.description41890
dc.description605
dc.description614
dc.languageen
dc.publisherEditions Scientifiques Medicales Elsevier
dc.publisherParis Cedex 15
dc.publisherFrança
dc.relationBiology Of The Cell
dc.relationBiol. Cell
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecthaemopoiesis
dc.subjectcytokines
dc.subjectglycosaminoglycans
dc.subjectFDC-P1 cells
dc.subjectS17 cells
dc.subjectliver connective tissue
dc.subjectColony-stimulating Factor
dc.subjectMurine Schistosomiasis-mansoni
dc.subjectHepatic Stellate Cells
dc.subjectHeparan-sulfate
dc.subjectInflammatory Reactions
dc.subjectMyeloid Proliferation
dc.subjectLiver Granulomas
dc.subjectMouse Cdna
dc.subjectProteoglycans
dc.subjectGranulocytopoiesis
dc.titleThe capacity of connective tissue stromas to sustain myelopoiesis depends both upon the growth factors and the local intercellular environment
dc.typeArtículos de revistas


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