Type II photooxidation mechanism of biomolecules using chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) as a photosensitizer

dc.creatorda Silva, AR
dc.creatorRibeiro, JN
dc.creatorRettori, D
dc.creatorJorge, RA
dc.date2008
dc.date2014-11-15T11:00:53Z
dc.date2015-11-26T17:19:38Z
dc.date2014-11-15T11:00:53Z
dc.date2015-11-26T17:19:38Z
dc.date.accessioned2018-03-29T00:07:17Z
dc.date.available2018-03-29T00:07:17Z
dc.identifierJournal Of The Brazilian Chemical Society. Soc Brasileira Quimica, v. 19, n. 7, n. 1311, n. 1320, 2008.
dc.identifier0103-5053
dc.identifierWOS:000259801100012
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76870
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/76870
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76870
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282947
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato) indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN(3). The presence of D(2)O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D(2)O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only (1)O(2) was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via (1)O(2) in biomolecule photooxidation by InTPP.
dc.description19
dc.description7
dc.description1311
dc.description1320
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherSoc Brasileira Quimica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationJournal Of The Brazilian Chemical Society
dc.relationJ. Braz. Chem. Soc.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectchloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
dc.subjectphotodynamic therapy
dc.subjectphotooxidation mechanism
dc.subjectcancer
dc.subjecterythrocytes
dc.subjectSensitized Photo-oxidation
dc.subjectExcited Singlet-state
dc.subjectPhotodynamic Therapy
dc.subjectMethyl Pyropheophorbide
dc.subjectMolecular-oxygen
dc.subjectCentral Metal
dc.subjectPhthalocyanine
dc.subjectCompetition
dc.subjectTryptamine
dc.subjectGeneration
dc.titleType II photooxidation mechanism of biomolecules using chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) as a photosensitizer
dc.typeArtículos de revistas


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