Targeted Antitumoral Dehydrocrotonin Nanoparticles With L-Ascorbic Acid 6-Stearate

dc.creatorFrungillo, L
dc.creatorMartins, D
dc.creatorTeixeira, S
dc.creatorAnazetti, MC
dc.creatorMelo, PD
dc.creatorDuran, N
dc.date2009
dc.dateDEC
dc.date2014-11-15T10:01:37Z
dc.date2015-11-26T17:19:25Z
dc.date2014-11-15T10:01:37Z
dc.date2015-11-26T17:19:25Z
dc.date.accessioned2018-03-29T00:07:05Z
dc.date.available2018-03-29T00:07:05Z
dc.identifierJournal Of Pharmaceutical Sciences. John Wiley & Sons Inc, v. 98, n. 12, n. 4796, n. 4807, 2009.
dc.identifier0022-3549
dc.identifierWOS:000273085400028
dc.identifier10.1002/jps.21760
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79045
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79045
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79045
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282901
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionTumoral cells are known to have a higher ascorbic acid uptake than normal cells. Therefore, the aim of this study was to obtain polymeric nanoparticles containing the antitumoral compound trans-dehydrocrotonin (DHC) functionalized with L-ascorbic acid 6-stearate (AAS) to specifically target this system tumoral cells. Nanoparticle suspensions (NP-AAS-DHC) were prepared by the nanoprecipitation method. The systems were characterized for AAS presence by thin-layer chromatography and for drug loading (81-88%) by UV-Vis spectroscopy. To further characterize these systems, in vitro release kinetics, size distribution (100-140 nm) and Zeta potential by photon-correlation spectroscopic method were used. In vitro toxicity against HL60 cells was evaluated by tetrazolium reduction and Trypan blue exclusion assays. Cell death by apoptosis was quantified and characterized by flow cytometry and caspase activity. Zeta potential analyses showed that the system has a negatively charged outer surface and also indicate that AAS is incorporated on the external surface of the nanoparticles. In vitro release kinetics assay showed that DHC loaded in nanoparticles had sustained release behavior. In vitro toxicity assays showed that NP-AAS-DHC suspension was more effective as an antitumoral than free DHC or NP-DHC and increased apoptosis induction by receptor-mediated pathway. (C) 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:4796-4807, 2009
dc.description98
dc.description12
dc.description4796
dc.description4807
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionMCT/PADCT/IMMP
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherJohn Wiley & Sons Inc
dc.publisherHoboken
dc.publisherEUA
dc.relationJournal Of Pharmaceutical Sciences
dc.relationJ. Pharm. Sci.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectcancer chemotherapy
dc.subjectcell culture
dc.subjectnanoparticles
dc.subjecttargeted drug delivery
dc.subjectdrug effects
dc.subjectCroton-cajucara
dc.subjectTrans-dehydrocrotonin
dc.subjectVitamin-c
dc.subjectDimethylamide-crotonin
dc.subjectLeukemia-cells
dc.subjectCancer-therapy
dc.subjectIn-vivo
dc.subjectDelivery
dc.subjectHl60
dc.subjectDiterpenes
dc.titleTargeted Antitumoral Dehydrocrotonin Nanoparticles With L-Ascorbic Acid 6-Stearate
dc.typeArtículos de revistas


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