Sugarcane yellow leaf disease in Brazil: Evidence of association with a luteovirus

dc.creatorVega, J
dc.creatorScagliusi, SMM
dc.creatorUlian, EC
dc.date1997
dc.dateJAN
dc.date2014-12-16T11:35:56Z
dc.date2015-11-26T17:19:00Z
dc.date2014-12-16T11:35:56Z
dc.date2015-11-26T17:19:00Z
dc.date.accessioned2018-03-29T00:06:41Z
dc.date.available2018-03-29T00:06:41Z
dc.identifierPlant Disease. Amer Phytopathological Soc, v. 81, n. 1, n. 21, n. 26, 1997.
dc.identifier0191-2917
dc.identifierWOS:A1997XN27600004
dc.identifier10.1094/PDIS.1997.81.1.21
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74721
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/74721
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74721
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282794
dc.descriptionEvidence of the viral etiology of sugarcane yellow leaf disease (SCYLD), occurring in southeast Brazil, was obtained by light and electron microscopy combined with serology. Light microscopy using epifluorescence illumination showed an abnormal yellow-green fluorescing material in the phloem of SCYLD-affected plants that was rarely observed in control plants. Immunolocalization in tissue-printed (or -blotted) nitrocellulose membranes, using barley yellow dwarf virus (BYDV) serotype PAV antiserum, showed a weak but clearly positive reaction in the phloem. Isometric viruslike particles of 24 to 26 nm in diameter were found by electron microscopy both in situ and in partially purified preparations. Examination of thin sections showed that phloem companion cells contained viruslike particles and presented cytological changes apparently related to the development of virus infection. Partially purified preparations produced UV absorption spectra typical of a nucleoprotein, with high absorbance at 260 nm, as expected for isometric virus particles. Virus particles were observed in extracts and partially purified preparations using immunosorbent electron microscopy with BYDV-PAV antiserum. Plate-trapped antigen enzyme-linked immunosorbent assays with the same antiserum indicate a weak serological relationship between BYDV-PAV and SCYLD-associated virus.
dc.description81
dc.description1
dc.description21
dc.description26
dc.languageen
dc.publisherAmer Phytopathological Soc
dc.publisherSt Paul
dc.relationPlant Disease
dc.relationPLANT DIS.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectautofluorescence
dc.subjectphloem-limited virus
dc.subjecttissue-printing immunohistochemistry
dc.subjectPlant-viruses
dc.subjectDwarf Virus
dc.subjectBarley
dc.titleSugarcane yellow leaf disease in Brazil: Evidence of association with a luteovirus
dc.typeArtículos de revistas


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