Molecular characterization of Brazilian avian pneumovirus isolates: comparison between immunochemiluminescent Southern blot and nested PCR

dc.creatorDani, MAC
dc.creatorDurigon, EL
dc.creatorArns, CW
dc.date1999
dc.dateMAY
dc.date2014-12-02T16:24:41Z
dc.date2015-11-26T17:18:36Z
dc.date2014-12-02T16:24:41Z
dc.date2015-11-26T17:18:36Z
dc.date.accessioned2018-03-29T00:06:18Z
dc.date.available2018-03-29T00:06:18Z
dc.identifierJournal Of Virological Methods. Elsevier Science Bv, v. 79, n. 2, n. 237, n. 241, 1999.
dc.identifier0166-0934
dc.identifierWOS:000080647000012
dc.identifier10.1016/S0166-0934(99)00020-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57737
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57737
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57737
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282700
dc.descriptionAvian pneumovirus (APV) causes acute respiratory tract infection both in turkeys (turkey rhinotracheitis) and chickens (swollen head syndrome (SHS)) with sudden onset and rapid spread through the flocks. In this study, an immunochemiluminescent Southern blot RT-PCR assay was employed to detect a F gene transcript of the APV in two European turkey isolates and two Brazilian chicken isolates. Limiting dilution PCR was carried out to compare the sensitivity of immunochemiluminescent Southern blot assay and nested PCR assay (nPCR). The sensitivity and specificity of immunochemiluminescent Southern blot RT-PCR assay were comparable to that of nPCR, and at least 100 fold more sensitive than a single PCR amplification. Sequence analysis of the 175 bp product of the F gene revealed 100% identity with APV sequences described earlier. (C) 1999 Elsevier Science B.V. All rights reserved.
dc.description79
dc.description2
dc.description237
dc.description241
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Virological Methods
dc.relationJ. Virol. Methods
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAPV
dc.subjectavian pneumovirus
dc.subjectpolymerase chain reaction
dc.subjectchemiluminescent detection
dc.subjectF gene
dc.subjectTurkey Rhinotracheitis Virus
dc.subjectPolymerase Chain-reaction
dc.subjectSwollen Head Syndrome
dc.subjectChickens
dc.subjectAntibodies
dc.subjectAssay
dc.subjectTrt
dc.titleMolecular characterization of Brazilian avian pneumovirus isolates: comparison between immunochemiluminescent Southern blot and nested PCR
dc.typeArtículos de revistas


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