Pouterin, a novel potential cytotoxic lectin-like protein with apoptosis-inducing activity in tumorigenic mammalian cells

dc.creatorBoleti, APD
dc.creatorVentura, CA
dc.creatorJusto, GZ
dc.creatorSilva, RA
dc.creatorde Sousa, ACT
dc.creatorFerreira, CV
dc.creatorYano, T
dc.creatorMacedo, MLR
dc.date2008
dc.date42156
dc.date2014-11-14T22:25:49Z
dc.date2015-11-26T17:17:31Z
dc.date2014-11-14T22:25:49Z
dc.date2015-11-26T17:17:31Z
dc.date.accessioned2018-03-29T00:05:23Z
dc.date.available2018-03-29T00:05:23Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 51, n. 8, n. 1321, n. 1330, 2008.
dc.identifier0041-0101
dc.identifierWOS:000257645200001
dc.identifier10.1016/j.toxicon.2008.03.007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81138
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/81138
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81138
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282467
dc.descriptionIn this study, the cytotoxicity of pouterin in tumorigenic and non-tumorigenic mammalian cell lines was investigated. We found that HeLa, Hep-2 and HT-29 tumor cells were highly sensitive to pouterin cytotoxicity in a dose-dependent manner, whereas non-tumorigenic Vero cells and human lymphocytes were relatively resistant to the protein. Among the tumor cell lines, HeLa cells showed the highest susceptibility to pouterin cytotoxicity, exhibiting a time-dependent increase in LDH leakage and an IC50 value of 5 mu g/mL. Morphological alterations such as rounding, cell shrinkage and chromatin condensation, consistent with apoptotic cell death were observed. Apoptosis induction was demonstrated by DNA fragmentation as detected by terminal dUTP nick-end labeling (TUNEL). Furthermore, HeLa cells incubated with pouterin showed disruption of the actin cytoskeleton. Western blot analysis revealed that pouterin caused increased expression of p21, thus indicating cell cycle arrest. Subsequent studies provided evidence that apoptosis may be partially explained in the activation of the tumor necrosis factor receptor 1 (TNFR1) signaling. Interestingly, a time-dependent decrease of the expression of p65 nuclear factor kappa B (NF kappa B) subunit, concomitant with a downregulation of the inhibitor of apoptosis protein 1 (IAP1) was observed, suggesting that TNFR-mediated apoptosis is the predominant pathway induced by pouterin in HeLa cells. (c) 2008 Elsevier Ltd. All rights reserved.
dc.description51
dc.description8
dc.description1321
dc.description1330
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectpouterin
dc.subjectHeLa cells
dc.subjectapoptosis
dc.subjectactin cytoskeleton
dc.subjectTNF receptor
dc.subjectNf-kappa-b
dc.subjectDna Fragmentation
dc.subjectTnf Receptor
dc.subjectCancer
dc.subjectActivation
dc.subjectMechanisms
dc.subjectAgglutinin
dc.subjectDeath
dc.subjectInduction
dc.subjectActin
dc.titlePouterin, a novel potential cytotoxic lectin-like protein with apoptosis-inducing activity in tumorigenic mammalian cells
dc.typeArtículos de revistas


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