Dynamics of polymorphism of acidocalcisomes in Leishmania parasites

dc.creatorMiranda, K
dc.creatorDocampo, R
dc.creatorGrillo, O
dc.creatorFranzen, A
dc.creatorAttias, M
dc.creatorVercesi, A
dc.creatorPlattner, H
dc.creatorHentschel, J
dc.creatorde Souza, W
dc.date2004
dc.dateMAY
dc.date2014-11-20T07:15:03Z
dc.date2015-11-26T17:17:27Z
dc.date2014-11-20T07:15:03Z
dc.date2015-11-26T17:17:27Z
dc.date.accessioned2018-03-29T00:05:19Z
dc.date.available2018-03-29T00:05:19Z
dc.identifierHistochemistry And Cell Biology. Springer-verlag, v. 121, n. 5, n. 407, n. 418, 2004.
dc.identifier0948-6143
dc.identifierWOS:000221643100007
dc.identifier10.1007/s00418-004-0646-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63619
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/63619
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63619
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282452
dc.descriptionGrowth of Leishmania mexicana amazonensis promastigotes in different culture media resulted in structurally and chemically different acidocalcisomes. When grown in SDM-79 medium, the promastigotes showed large spherical acidocalcisomes of up to 1.2 mum diameter distributed throughout the cell. X-ray microanalysis and elemental mapping of the organelles showed large amounts of oxygen, phosphorus, sodium, potassium, magnesium, calcium, and zinc. Immunofluorescence microscopy using antisera raised against a peptide sequence of the vacuolar-type proton pyrophosphatase of Arabidopsis thaliana that is conserved in the Leishmania enzyme, indicated localization in acidocalcisomes. When cells were transferred to Warren's medium, the acidocalcisomes transformed from spherical into branched tubular organelles. The labeling pattern of the vacuolar proton-pyrophosphatase, considered as a marker for the organelle, changed accompanying the structural changes of the acidocalcisomes, and the enzyme showed an apparently lower proton-transporting activity when measured in digitonin-permeabilized promastigotes. X-ray microanalysis and elemental mapping of these structures revealed the additional presence of iron. Together, the results reveal that the morphology and composition of acidocalcisomes are greatly influenced by the culture conditions.
dc.description121
dc.description5
dc.description407
dc.description418
dc.languageen
dc.publisherSpringer-verlag
dc.publisherNew York
dc.publisherEUA
dc.relationHistochemistry And Cell Biology
dc.relationHistochem. Cell Biol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectLeishmania amazonensis
dc.subjectacidocalcisomes
dc.subjectelemental mapping
dc.subjectvacuolar proton pyrophosphatase
dc.subjectiron uptake
dc.subjectProton-pumping Pyrophosphatase
dc.subjectTrypanosoma-cruzi
dc.subjectSpecial Organelles
dc.subjectNa+/h+ Exchanger
dc.subjectCa2+ Release
dc.subjectH+-atpase
dc.subjectBrucei
dc.subjectLocalization
dc.subjectStorage
dc.subjectDifferentiation
dc.titleDynamics of polymorphism of acidocalcisomes in Leishmania parasites
dc.typeArtículos de revistas


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