Bowman-Birk and Kunitz Protease Inhibitors among Antinutrients and Bioactives Modified by Germination and Hydrolysis in Brazilian Soybean Cultivar BRS 133

dc.creatorDia, VP
dc.creatorGomez, T
dc.creatorVernaza, G
dc.creatorBerhow, M
dc.creatorChang, YK
dc.creatorde Mejia, EG
dc.date2012
dc.dateAUG 15
dc.date2014-11-14T21:28:14Z
dc.date2015-11-26T17:17:14Z
dc.date2014-11-14T21:28:14Z
dc.date2015-11-26T17:17:14Z
dc.date.accessioned2018-03-29T00:05:12Z
dc.date.available2018-03-29T00:05:12Z
dc.identifierJournal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 60, n. 32, n. 7886, n. 7894, 2012.
dc.identifier0021-8561
dc.identifier1520-5118
dc.identifierWOS:000307487100017
dc.identifier10.1021/jf301926w
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78241
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78241
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78241
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282425
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSoybean contains constituents that have antinutritional and bioactive properties. Enzymatic hydrolysis and germination can enhance the biological activity of these compounds in soybean. The objective of this study was to investigate the effect of germination, Alcalase (protease) hydrolysis, and their combination on the concentrations of antinutritional and bioactive compounds in Brazilian soybean cultivar BRS 133. A combination of germination and Alcalase hydrolysis resulted in the degradation of Bowman-Birk inhibitor (BBI), Kunitz trypsin inhibitor (KTI), and lunasin by 96.9, 97.8, and 38.4%. Lectin was not affected by any of the processing treatments when compared to nongerminated and nonhydrolyzed soy protein extract. Total isoflavones (ISF) and total saponins (SAP) increased by 16.2 and 28.7%, respectively, after 18 h of germination, while Alcalase hydrolysis led to the reduction of these compounds. A significant correlation was found between concentrations of BBI and KTI, BBI and lunasin, BBI and ISF, KTI and lunasin, KTI and ISF, KTI and SAP, lunasin and ISF, and ISF and SAP. Germination and Alcalase hydrolysis interacted in reducing BBI, ISF, and SAP. This study presents a process of preparing soy flour ingredients with lower concentrations of antinutritional factors and with biologically active constituents, important for the promotion of health associated with soybean consumption. In conclusion, 18 h of germination and 3 h of Alcalase hydrolysis is recommended for elimination of protease inhibitors, while bioactives are maintained by at least 50% of their original concentrations.
dc.description60
dc.description32
dc.description7886
dc.description7894
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherAmer Chemical Soc
dc.publisherWashington
dc.publisherEUA
dc.relationJournal Of Agricultural And Food Chemistry
dc.relationJ. Agric. Food Chem.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectsoybean
dc.subjectgermination
dc.subjectAlcalase hydrolysis
dc.subjectantinutritional factors
dc.subjectbioactive compounds
dc.subjectGlycine-max Germination
dc.subjectIn-vitro
dc.subjectChemical-composition
dc.subjectAntinutritional Factors
dc.subjectNutritional Factors
dc.subjectGamma-irradiation
dc.subjectDigestibility
dc.subjectSeeds
dc.subjectAntioxidant
dc.subjectAgglutinin
dc.titleBowman-Birk and Kunitz Protease Inhibitors among Antinutrients and Bioactives Modified by Germination and Hydrolysis in Brazilian Soybean Cultivar BRS 133
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución