Saci-1, -2, and -3 and Perere, four novel retrotransposons with high transcriptional activities from the human parasite Schistosoma mansoni

dc.creatorDeMarco, R
dc.creatorKowaltowski, AT
dc.creatorMachado, AA
dc.creatorSoares, MB
dc.creatorGargioni, C
dc.creatorKawano, T
dc.creatorRodrigues, V
dc.creatorMadeira, AMBN
dc.creatorWilson, RA
dc.creatorMenck, CFM
dc.creatorSetubal, JC
dc.creatorDias-Neto, E
dc.creatorLeite, LCC
dc.creatorVerjovski-Almeida, S
dc.date2004
dc.dateMAR
dc.date2014-11-20T07:01:39Z
dc.date2015-11-26T17:16:59Z
dc.date2014-11-20T07:01:39Z
dc.date2015-11-26T17:16:59Z
dc.date.accessioned2018-03-29T00:05:08Z
dc.date.available2018-03-29T00:05:08Z
dc.identifierJournal Of Virology. Amer Soc Microbiology, v. 78, n. 6, n. 2967, n. 2978, 2004.
dc.identifier0022-538X
dc.identifierWOS:000220043100031
dc.identifier10.1128/JVI.78.6.2967-2978.2004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71613
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71613
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71613
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282404
dc.descriptionUsing the data set of 180,000 expressed sequence tags (ESTs) of the blood fluke Schistosoma mansoni generated recently by our group, we identified three novel long-terminal-repeat (LTR)- and one novel non-LTR-expressed retrotransposon, named Saci-1, -2, and -3 and Perere, respectively. Full-length sequences were reconstructed from ESTs and have deduced open reading frames (ORFs) with several uncorrupted features, characterizing them as possible active retrotransposons of different known transposon families. Alignment of reconstructed sequences to available preliminary genome sequence data confirmed the overall structure of the transposons. The frequency of sequenced transposon transcripts in cercariae was 14% of all transcripts from that stage, twofold higher than that in schistosomula and three- to fourfold higher than that in adults, eggs, miracidia, and germ balls. We show by Southern blot analysis, by EST annotation and tallying, and by counting transposon tags from a Social Analysis of Gene Expression library, that the four novel retrotransposons exhibit a 10- to 30-fold lower copy number in the genome and a 4- to 200-fold-higher transcriptional rate per copy than the four previously described S. mansoni retrotransposons. Such differences lead us to hypothesize that there are two different populations of retrotransposons in S. mansoni genome, occupying different niches in its ecology. Examples of retrotransposon fragment inserts were found into the 5' and 3' untranslated regions of four different S. mansoni target gene transcripts. The data presented here suggest a role for these elements in the dynamics of this complex human parasite genome.
dc.description78
dc.description6
dc.description2967
dc.description2978
dc.languageen
dc.publisherAmer Soc Microbiology
dc.publisherWashington
dc.publisherEUA
dc.relationJournal Of Virology
dc.relationJ. Virol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectLong Terminal Repeat
dc.subjectPao-like Retrotransposons
dc.subjectTransposable Elements
dc.subjectDrosophila-melanogaster
dc.subjectBlood Fluke
dc.subjectEndogenous Retroviruses
dc.subjectCytosine Methylation
dc.subjectLtr Retrotransposons
dc.subjectDna
dc.subjectGene
dc.titleSaci-1, -2, and -3 and Perere, four novel retrotransposons with high transcriptional activities from the human parasite Schistosoma mansoni
dc.typeArtículos de revistas


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