dc.creatorKowaltowski, AJ
dc.creatorNaia-da-Silva, ES
dc.creatorCastilho, RF
dc.creatorVercesi, AE
dc.date1998
dc.date37196
dc.date2014-12-02T16:29:44Z
dc.date2015-11-26T17:16:23Z
dc.date2014-12-02T16:29:44Z
dc.date2015-11-26T17:16:23Z
dc.date.accessioned2018-03-29T00:04:35Z
dc.date.available2018-03-29T00:04:35Z
dc.identifierArchives Of Biochemistry And Biophysics. Academic Press Inc, v. 359, n. 1, n. 77, n. 81, 1998.
dc.identifier0003-9861
dc.identifierWOS:000076635900011
dc.identifier10.1006/abbi.1998.0870
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78264
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78264
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78264
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282272
dc.descriptionMitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and t-butyl hydroperoxide or inorganic phosphate, but not 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid or phenylarsine oxide, are inhibited by the local anesthetic dibucaine, Dibucaine promotes an inhibition of the Ca2+-induced increase in mitochondrial H2O2 generation measured by the oxidation of scopoletin in the presence of horseradish peroxidase, This decrease in mitochondrial H2O2 generation may be attributed to the reduction of Ca2+ binding to the membrane induced by dibucaine, as assessed by measuring Ca-45(2+) binding to the mitochondrial membrane. Mg2+ also inhibited Ca2+ binding to the mitochondrial membrane, mitochondrial swelling, membrane protein thiol oxidation, and H2O2 generation induced by Ca2+. Together, these results demonstrate that the mechanism by which dibucaine and Mg2+ inhibit mitochondrial permeability transition is related to the decrease in reactive oxygen species generation induced by Ca2+-promoted alterations of inner mitochondrial membrane properties. (C) 1998 Academic Press.
dc.description359
dc.description1
dc.description77
dc.description81
dc.languageen
dc.publisherAcademic Press Inc
dc.publisherSan Diego
dc.publisherEUA
dc.relationArchives Of Biochemistry And Biophysics
dc.relationArch. Biochem. Biophys.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcalcium
dc.subjectdibucaine
dc.subjectMg2+
dc.subjectmitochondrial permeability transition
dc.subjectreactive oxygen species
dc.subjectliver mitochondria
dc.subjectHydrogen-peroxide Formation
dc.subjectRat-liver Mitochondria
dc.subjectThiol Cross-linking
dc.subjectLocal-anesthetics
dc.subjectPlus Prooxidants
dc.subjectCa2+ Ions
dc.subjectMembrane
dc.subjectCyclosporine
dc.subjectApoptosis
dc.subjectPore
dc.titleCa2+-stimulated mitochondrial reactive oxygen species generation and permeability transition are inhibited by dibucaine or Mg2+
dc.typeArtículos de revistas


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