Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5

dc.creatorD'Arce, RCF
dc.creatorAlmeida, RS
dc.creatorSilva, TC
dc.creatorFranco, AC
dc.creatorSpilki, F
dc.creatorRoehe, PM
dc.creatorArns, CW
dc.date2002
dc.dateSEP 24
dc.date2014-11-20T06:10:17Z
dc.date2015-11-26T17:16:01Z
dc.date2014-11-20T06:10:17Z
dc.date2015-11-26T17:16:01Z
dc.date.accessioned2018-03-29T00:04:14Z
dc.date.available2018-03-29T00:04:14Z
dc.identifierVeterinary Microbiology. Elsevier Science Bv, v. 88, n. 4, n. 315, n. 324, 2002.
dc.identifier0378-1135
dc.identifierWOS:000178054100002
dc.identifier10.1016/S0378-1135(02)00126-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71068
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71068
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71068
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282186
dc.descriptionTwelve Brazilian isolates and three reference strains of bovine herpesviruses (BHVs) were subjected to restriction endonuclease analysis (REA) and monoclonal antibody (MAb) analysis. Viral DNA was cleaved with BamHI, BstEII, EcoRI, HindIII and PstI. The monoclonal antibody panel allowed the differentiation between types I and 5 viruses, while REA with BstEII and HindIII showed the distinction between BHV-1 and -5 subtypes. Typical 1.1 and 1.2a patterns were observed with two isolates from respiratory disease. An isolate from semen of a clinically healthy bull displayed 1.2b, profile, whereas another displayed a clear 5a pattern, which was never reported before. Seven out of nine Brazilian type 5 (BHV-5) isolates displayed REA patterns similar to the Australian BHV-5 strain N569 (BHV-5a), and differing from the Argentinean A663 strain (BHV-5b) virus. Another two BHV-5 isolates, which displayed an unusual MAb pattern of reactivity, showed a BstEII profile different from both reference strains of BHV-5. These two viruses were considered BHV-5 "non-a/non-b" subtype. (C) 2002 Elsevier Science B.V. All rights reserved.
dc.description88
dc.description4
dc.description315
dc.description324
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectbovine herpesvirus type 1 (BHV-1)
dc.subjectbovine herpesvirus type 5 (BHV-5)
dc.subjectrestriction endonuclease analysis
dc.subjectmonoclonal antibodies
dc.subjectEncephalitis
dc.subjectStrains
dc.subjectGenome
dc.subjectBhv-1
dc.subjectVirus
dc.subjectCalves
dc.titleRestriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5
dc.typeArtículos de revistas


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