Circadian Rhythm and Cartilage Extracellular Matrix Genes in Osseointegration: A Genome-Wide Screening of Implant Failure by Vitamin D Deficiency

dc.creatorMengatto, CM
dc.creatorMussano, F
dc.creatorHonda, Y
dc.creatorColwell, CS
dc.creatorNishimura, I
dc.date2011
dc.date40544
dc.date2014-07-30T13:59:35Z
dc.date2015-11-26T17:15:57Z
dc.date2014-07-30T13:59:35Z
dc.date2015-11-26T17:15:57Z
dc.date.accessioned2018-03-29T00:04:11Z
dc.date.available2018-03-29T00:04:11Z
dc.identifierPlos One. Public Library Science, v. 6, n. 1, 2011.
dc.identifier1932-6203
dc.identifierWOS:000286514000007
dc.identifier10.1371/journal.pone.0015848
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55931
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55931
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282172
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBackground: Successful dental and orthopedic implants require the establishment of an intimate association with bone tissue; however, the mechanistic explanation of how biological systems accomplish osseointegration is still incomplete. We sought to identify critical gene networks involved in osseointegration by exploring the implant failure model under vitamin D deficiency. Methodology: Adult male Sprague-Dawley rats were exposed to control or vitamin D-deficient diet prior to the osteotomy surgery in the femur bone and the placement of T-shaped Ti4Al6V implant. Two weeks after the osteotomy and implant placement, tissue formed at the osteotomy site or in the hollow chamber of T-shaped implant was harvested and total RNA was evaluated by whole genome microarray analyses. Principal Findings: Two-way ANOVA of microarray data identified 103 genes that were significantly (. 2 fold) modulated by the implant placement and vitamin D deficiency. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses assigned the highest z-score to the circadian rhythm pathway including neuronal PAS domain 2 (NPAS2), and period homolog 2 (Per2). NPAS2 and Aryl hydrocarbon receptor nuclear translocator-like (ARNTL/Bmal 1) were upregulated around implant and diminished by vitamin D deficiency, whereas the expression pattern of Per2 was complementary. Hierarchical cluster analysis further revealed that NPAS2 was in a group predominantly composed of cartilage extracellular matrix (ECM) genes. Whereas the expression of bone ECM genes around implant was not significantly affected by vitamin D deficiency, cartilage ECM genes were modulated by the presence of the implant and vitamin D status. In a proof-of-concept in vitro study, the expression of cartilage type II and X collagens was found upregulated when mouse mesenchymal stem cells were cultured on implant disk with 1,25D supplementation. Conclusions: This study suggests that the circadian rhythm system and cartilage extracellular matrix may be involved in the establishment of osseointegration under vitamin D regulation.
dc.description6
dc.description1
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBiomet3I, Palm Beach Garden, FL
dc.descriptionNational Center for Research Resources, National Institutes of Health (NCRR/NIH) [C06 RR014529]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionNational Center for Research Resources, National Institutes of Health (NCRR/NIH) [C06 RR014529]
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectBone
dc.subjectClock
dc.subjectChondrogenesis
dc.subjectMicroarray
dc.subjectSurfaces
dc.subjectCalcium
dc.subjectHealth
dc.subjectMouse
dc.subjectTranscription
dc.subjectChondrocyte
dc.titleCircadian Rhythm and Cartilage Extracellular Matrix Genes in Osseointegration: A Genome-Wide Screening of Implant Failure by Vitamin D Deficiency
dc.typeArtículos de revistas


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