Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture

dc.creatorDuarte, ADS
dc.creatorSales, TSI
dc.creatorMengel, JO
dc.creatorCosta, FF
dc.creatorSaad, STO
dc.date2002
dc.dateSEP-OCT
dc.date2014-11-14T11:52:48Z
dc.date2015-11-26T17:14:49Z
dc.date2014-11-14T11:52:48Z
dc.date2015-11-26T17:14:49Z
dc.date.accessioned2018-03-29T00:03:06Z
dc.date.available2018-03-29T00:03:06Z
dc.identifierBlood Cells Molecules And Diseases. Academic Press Inc Elsevier Science, v. 29, n. 2, n. 213, n. 224, 2002.
dc.identifier1079-9796
dc.identifierWOS:000179991100009
dc.identifier10.1006/bcmd.2002.0551
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70475
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70475
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70475
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281897
dc.descriptionSteroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects. (C) 2002 Elsevier Science (USA).
dc.description29
dc.description2
dc.description213
dc.description224
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationBlood Cells Molecules And Diseases
dc.relationBlood Cells Mol. Dis.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectHbF
dc.subjectprogesterone
dc.subjecttranscription factors
dc.subjectRT-PCR
dc.subjectgene expression
dc.subjectReceptor Coactivator-1 Src-1
dc.subjectGlobin Gene-expression
dc.subjectMegakaryocytic Differentiation
dc.subjectTranscription Factors
dc.subjectFetal Hemoglobin
dc.subjectHematopoiesis
dc.subjectHydroxyurea
dc.subjectMaturation
dc.subjectChromatin
dc.subjectInducers
dc.titleProgesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture
dc.typeArtículos de revistas


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