Differentiation of Xanthomonas species by PCR-RFLP of rpfB and atpD genes

dc.creatorSimoes, THN
dc.creatorGoncalves, ER
dc.creatorRosato, YB
dc.creatorMehta, A
dc.date2007
dc.dateJUN
dc.date2014-11-14T08:29:23Z
dc.date2015-11-26T17:14:02Z
dc.date2014-11-14T08:29:23Z
dc.date2015-11-26T17:14:02Z
dc.date.accessioned2018-03-29T00:02:23Z
dc.date.available2018-03-29T00:02:23Z
dc.identifierFems Microbiology Letters. Blackwell Publishing, v. 271, n. 1, n. 33, n. 39, 2007.
dc.identifier0378-1097
dc.identifierWOS:000246241500006
dc.identifier10.1111/j.1574-6968.2007.00691.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61828
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61828
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61828
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281716
dc.descriptionThe genetic characterization of Xanthomonas species remains a challenge. Several DNA-based techniques have been previously employed, including the analysis of the 16S rRNA and 16S-23S rRNA genes in order to differentiate and classify the Xanthomonas species. However, several species could not be distinguished in these studies, due to the high degree of conservation of these molecular markers. In order to obtain more efficient markers, and to better understand the phylogenetic relationships between the Xanthomonas species, two genes commonly found in the different species have been analyzed. The genes rpfB and atpD involved in the regulation of pathogenicity factors and in the synthesis of ATP, respectively, were amplified in Xanthomonas species and further analyzed by PCR-restriction fragment length polymorphism. Dendrograms with the data sets of the rpfB and atpD analyzed separately and combined were constructed. The results obtained revealed that several Xanthomonas species, previously grouped together, could be successfully distinguished using these markers. The results obtained herein provide an alternative method for the distinction of the Xanthomonas species and contribute to a better understanding of the genetic and phylogenetic relationships of Xanthomonas.
dc.description271
dc.description1
dc.description33
dc.description39
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationFems Microbiology Letters
dc.relationFEMS Microbiol. Lett.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectXanthomonas
dc.subjectgenetic diversity
dc.subjectphylogenetic analysis
dc.subjectMolecular Systematics
dc.subjectSequence Alignment
dc.subjectGenus Xanthomonas
dc.subjectPseudomonas
dc.subjectGenetics
dc.subjectStrains
dc.titleDifferentiation of Xanthomonas species by PCR-RFLP of rpfB and atpD genes
dc.typeArtículos de revistas


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