Separation of fructooligosaccharides using zeolite fixed bed columns

dc.creatorKuhn, RC
dc.creatorMaugeri, F
dc.date2010
dc.date42186
dc.date2014-11-14T07:24:19Z
dc.date2015-11-26T17:13:47Z
dc.date2014-11-14T07:24:19Z
dc.date2015-11-26T17:13:47Z
dc.date.accessioned2018-03-29T00:02:08Z
dc.date.available2018-03-29T00:02:08Z
dc.identifierJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 878, n. 22, n. 2023, n. 2028, 2010.
dc.identifier1570-0232
dc.identifierWOS:000280210300017
dc.identifier10.1016/j.jchromb.2010.05.039
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/75634
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/75634
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/75634
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281655
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionRecent studies have shown that the chromatographic separation of mixtures of monosaccharides and disaccharides may be improved by employing Y zeolites, a procedure which holds promise in the separation of oligosaccharides. In the present study, a column packed with zeolite was employed to study the separation of fructooligosaccharides (FOS). FOS were produced by an enzyme isolated from Rhodotorula sp., which produces GF(2) (kestose), GF(3) (nystose) and GF(4) (frutofuranosyl nystose). The identification and quantification of the sugars were carried out by ion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The separation of fructooligosaccharides was carried out using a fixed bed column packed with Ba(2+)-exchange Y zeolites. The effects of temperature (40-50 degrees C), injected volume per bed volume (2.55-7.64%), superficial velocity (0.1-0.15 cm min(-1)) and eluent composition (40-60% ethanol) were investigated using a fractionary factorial design with separation efficiency as the response. The results showed that the most favorable conditions for the separation of the oligosaccharide-glucose mixture were 60% ethanol as eluent, temperature of 50 degrees C, superficial velocity of 0.1 cm min(-1) and 2.55% injection volume per bed volume of injection mixture, using two columns in series. The values for separation efficiency were 0.60 for oligosaccharide-glucose, 1.00 for oligosaccharide-fructose, 0.22 for oligosaccharide-sucrose, 0.43 for glucose-fructose, 0.82 for glucose-sucrose and 1.23 for fructose-sucrose. (C) 2010 Elsevier B.V. All rights reserved.
dc.description878
dc.description22
dc.description2023
dc.description2028
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences
dc.relationJ. Chromatogr. B
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectFixed bed column
dc.subjectFructooligosaccharides
dc.subjectZeolites
dc.subjectSeparation
dc.subjectExperimental design
dc.subjectAdsorption
dc.subjectMixed-enzyme-system
dc.subjectBeta-fructofuranosidase
dc.subjectFructo-oligosaccharides
dc.subjectAqueous-solutions
dc.subjectGlucose-oxidase
dc.subjectFructosyltransferase
dc.subjectNanofiltration
dc.subjectPurification
dc.subjectAdsorption
dc.subjectRemoval
dc.titleSeparation of fructooligosaccharides using zeolite fixed bed columns
dc.typeArtículos de revistas


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