Organization of collagen bundles during tendon healing in rats treated with L-NAME

dc.creatorTomiosso, TC
dc.creatorNakagaki, WR
dc.creatorGomes, L
dc.creatorHyslop, S
dc.creatorPimentel, ER
dc.date2009
dc.dateAUG
dc.date2014-11-14T04:17:15Z
dc.date2015-11-26T17:13:13Z
dc.date2014-11-14T04:17:15Z
dc.date2015-11-26T17:13:13Z
dc.date.accessioned2018-03-29T00:01:35Z
dc.date.available2018-03-29T00:01:35Z
dc.identifierCell And Tissue Research. Springer, v. 337, n. 2, n. 235, n. 242, 2009.
dc.identifier0302-766X
dc.identifierWOS:000268422800005
dc.identifier10.1007/s00441-009-0819-5
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69742
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69742
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69742
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281516
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe Achilles tendon can support high tension forces and may experience lesions. The damaged tissue does not regenerate completely, with the organization and mechanical properties of the repaired tendon being inferior to those of a healthy tendon. Nitric oxide (NO) plays an important role in wound repair. We have examined the structural reorganization and repair in Achilles tendon after injury in rats treated with the NO synthase inhibitor L-NAME. The right Achilles tendon of male Wistar rats was partially transected. One group of rats was treated with L-NAME (similar to 300 mg/kg per day, given in drinking water) for 4 days prior to tendon sectioning and throughout the post-operative period. Control rats received water without L-NAME. The tendons were excised at 7, 14, and 21 days post-injury and used to quantify hydroxyproline and for mechanical tests. Tendons were also processed for histomorphological analysis by polarized light microscopy, which showed that the collagen fibers were disorganized by day 7 in non-treated and L-NAME-treated rats. In non-treated rats, the organization of the extracellular matrix was more homogeneous by days 14 and 21 compared with day 7, although this homogeneity was less than that in normal tendon. In contrast, in injured tendons from L-NAME-treated rats, the collagen fibers were still disorganized on day 21. Tendons from treated rats had more hydroxyproline but lower mechanical properties compared with those from non-treated rats. Thus, NO modulates tendon healing, with a reduction in NO biosynthesis delaying reorganization of the extracellular matrix, especially collagen.
dc.description337
dc.description2
dc.description235
dc.description242
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationCell And Tissue Research
dc.relationCell Tissue Res.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectBiomechanics
dc.subjectCollagen
dc.subjectTendon
dc.subjectL-NAME
dc.subjectNitric oxide
dc.subjectRat (Wistar, male)
dc.subjectNitric-oxide Synthesis
dc.subjectAchilles-tendon
dc.subjectBiomechanical Properties
dc.subjectTemporal Expression
dc.subjectSynthase Gene
dc.subjectKnockout Mice
dc.subjectLigament
dc.subjectWounds
dc.subjectInjury
dc.subjectRepair
dc.titleOrganization of collagen bundles during tendon healing in rats treated with L-NAME
dc.typeArtículos de revistas


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