Interaction of histidine-tagged human proinsulin with immobilized nickel ion: Effect of chelating ligand and thermodynamics analysis

dc.creatorde Goes, LC
dc.creatorMiranda, EA
dc.creatorBueno, SMA
dc.date2010
dc.dateOCT 20
dc.date2014-11-20T03:06:28Z
dc.date2015-11-26T17:12:53Z
dc.date2014-11-20T03:06:28Z
dc.date2015-11-26T17:12:53Z
dc.date.accessioned2018-03-29T00:01:17Z
dc.date.available2018-03-29T00:01:17Z
dc.identifierColloids And Surfaces A-physicochemical And Engineering Aspects. Elsevier Science Bv, v. 369, n. 41699, n. 176, n. 185, 2010.
dc.identifier0927-7757
dc.identifierWOS:000283395600025
dc.identifier10.1016/j.colsurfa.2010.08.019
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60366
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60366
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60366
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281440
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThe nature of the chelating ligand is one of various factors affecting the performance of IMAC. In this work we studied the effect of three chelating ligands on the adsorption of recombinant human proinsulin with His-tag (proinsulin-(His)(6)). The chelating ligands (complexed with Ni(II)) were two tetradentates (carboxymethylated aspartic acid, CM-Asp, and tris(2-aminoethyl)amine, TREN) and one pentadentate (tris (carboxymethyl) ethylenediamine, TED). Their performance were compared with the performance of one tridentate ligand (iminodiacetic acid, IDA) and the commercial adsorbent HisTrap both also complexed with Ni(11). Higher selectivities were achieved with Ni(II)-CM-Asp and Ni(II)-TED. The adsorption capacity decreased according to the order: TREN, HisTrap, IDA, CM-Asp, and TED (172.82, 167.98, 133.09, 110.18, and 69.22 mg of proinsulin/mL of gel, respectively, at 25 degrees C). Although TREN-agarose had the highest capacity, the ligand with the second highest capacity - HisTrap - showed better performance since proinsulin was eluted in a single, concentrated peak with imidazole, while for Ni(II)-TREN proinsulin was eluted with imidazole and EDTA, requiring an extra step to free the product from the EDTA. Therefore, the choice of an adsorbent for this separation depends on the priority: capacity (TREN or HisTrap for one step process) or selectivity (CM-Asp). Adsorption isotherms were determined for temperatures from 4 to 45 degrees C and the Langmuir model was fitted to the experimental data. The thermodynamics analysis showed positive Delta H(o) (from 18.83 to 86.05 kJ/mol), indicating that the adsorption of proinsuin-(His)(6) in all chelating ligands is an endothermic process. The negative change in Gibbs free energy (from -26.30 to -35.11 kJ/mol) indicated that the adsorption of the proinsulin-(His)(6) on all the adsorbents studied was a thermodynamically favorable process. (C) 2010 Elsevier B.V. All rights reserved.
dc.description369
dc.description41699
dc.description176
dc.description185
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationColloids And Surfaces A-physicochemical And Engineering Aspects
dc.relationColloid Surf. A-Physicochem. Eng. Asp.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectIMAC
dc.subjectIsotherms
dc.subjectRecombinant proinsulin
dc.subjectAdsorption
dc.subjectThermodynamic parameters
dc.subjectAffinity-chromatography Imac
dc.subjectRecombinant Proteins
dc.subjectLarge-scale
dc.subjectMetal
dc.subjectPurification
dc.subjectTags
dc.subjectAdsorption
dc.subjectLysozyme
dc.subjectStrength
dc.subjectBinding
dc.titleInteraction of histidine-tagged human proinsulin with immobilized nickel ion: Effect of chelating ligand and thermodynamics analysis
dc.typeArtículos de revistas


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