Glutaraldehyde-treated bovine pericardium: Effects of lyophilization on cytotoxicity and residual aldehydes

dc.creatorMaizato, MJS
dc.creatorHiga, OZ
dc.creatorMathor, MB
dc.creatorCamillo, MAP
dc.creatorSpencer, PJ
dc.creatorPitombo, RN
dc.creatorZavaglia, CAC
dc.creatorLeirner, AA
dc.date2003
dc.dateAUG
dc.date2014-11-20T02:31:27Z
dc.date2015-11-26T17:12:23Z
dc.date2014-11-20T02:31:27Z
dc.date2015-11-26T17:12:23Z
dc.date.accessioned2018-03-29T00:00:48Z
dc.date.available2018-03-29T00:00:48Z
dc.identifierArtificial Organs. Wiley-blackwell, v. 27, n. 8, n. 692, n. 694, 2003.
dc.identifier0160-564X
dc.identifierWOS:000184720100005
dc.identifier10.1046/j.1525-1594.2003.07275.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68114
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/68114
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68114
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281316
dc.descriptionThis work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentra- tion of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular growth. Residual aldehydes were measured by HPLC. Although both groups' eluants exhibited some cytotoxicity, the eluant from group A was less cytotoxic, with a cytotoxicity index (IC(50(%)) ) of 41%. Group B eluants all had marked cytotoxic effects; cell growth was 24.15% of the negative control at the most dilute eluant concentration (6.25%). The mean residual glutaraldehyde level was less in group A than in group B (2.36 +/- 0.11 and 9.90 +/- 3.70 g/l, respectively; n=3, P < 0.05), but residual formaldehyde levels did not differ. These results demonstrate that compared with conventional glutaraldehyde-treated bovine pericardium, lyophilized pericardium is less cytotoxic, with fewer glutaraldehyde residues.
dc.description27
dc.description8
dc.description692
dc.description694
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationArtificial Organs
dc.relationArtif. Organs
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectbiocompatibility
dc.subjectcytotoxicity
dc.subjectbioprosthesis
dc.subjectbovine pericardium
dc.subjectglutaraldehyde
dc.subjectFixed Pericardium
dc.subjectBioprostheses
dc.subjectTissue
dc.titleGlutaraldehyde-treated bovine pericardium: Effects of lyophilization on cytotoxicity and residual aldehydes
dc.typeArtículos de revistas


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