The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

dc.creatorStehling, EG
dc.creatorCampos, TA
dc.creatorBrocchil, M
dc.creatorAzevedo, VAD
dc.creatorda Silveira, WD
dc.date2008
dc.dateMAR
dc.date2014-11-20T01:53:40Z
dc.date2015-11-26T17:11:49Z
dc.date2014-11-20T01:53:40Z
dc.date2015-11-26T17:11:49Z
dc.date.accessioned2018-03-29T00:00:17Z
dc.date.available2018-03-29T00:00:17Z
dc.identifierJournal Of Veterinary Science. Korean Soc Veterinary Science, v. 9, n. 1, n. 75, n. 83, 2008.
dc.identifier1229-845X
dc.identifierWOS:000253787300010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73838
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73838
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73838
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281188
dc.descriptionEscherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 x 10(5) CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 x 10(12) CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was transferred, by electroporation assay, to non-pathogenic receptor strains (E. coli strains K12 MS101 and HB101), resulting in transformant strains A and R, respectively. These strains exhibited adhesion properties to in vitro cultivated HEp-2 cells but did not have the capacity for invasion. The adherence occurred despite the absence of fimbriae; this finding suggests that the 88 MDa plasmid has afimbrial adhesin genes.
dc.description9
dc.description1
dc.description75
dc.description83
dc.languageen
dc.publisherKorean Soc Veterinary Science
dc.publisherSeoul
dc.publisherCoreia do Sul
dc.relationJournal Of Veterinary Science
dc.relationJ. Vet. Sci.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectadhesion
dc.subjectavian
dc.subjectEscherichia coli
dc.subjectplasmids
dc.subjectPolymerase-chain-reaction
dc.subjectVirulence Factors
dc.subjectMolecular Characterization
dc.subjectEncoding Operons
dc.subjectIn-vitro
dc.subjectIdentification
dc.subjectChickens
dc.subjectCells
dc.subjectHemagglutination
dc.subjectResistance
dc.titleThe expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
dc.typeArtículos de revistas


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