Brasil | Artículos de revistas

Extracts from Alternanthera maritima as natural photosensitizers in photodynamic antimicrobial chemotherapy (PACT)

dc.creatorGasparetto, A
dc.creatorLapinski, TF
dc.creatorZamuner, SR
dc.creatorKhouri, S
dc.creatorAlves, LP
dc.creatorMunin, E
dc.creatorSalvador, MJ
dc.date2010
dc.dateAPR 2
dc.date2014-11-13T21:32:48Z
dc.date2015-11-26T17:11:41Z
dc.date2014-11-13T21:32:48Z
dc.date2015-11-26T17:11:41Z
dc.date.accessioned2018-03-29T00:00:09Z
dc.date.available2018-03-29T00:00:09Z
dc.identifierJournal Of Photochemistry And Photobiology B-biology. Elsevier Science Sa, v. 99, n. 1, n. 15, n. 20, 2010.
dc.identifier1011-1344
dc.identifierWOS:000276822000003
dc.identifier10.1016/j.jphotobiol.2010.01.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66600
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66600
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66600
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281155
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis study investigated the effect of photodynamic antimicrobial chemotherapy (PACT) using extracts from Alternanthera maritima on the viability of Candida dubliniensis. Human infections constitute a great health problem. Several antifungal drugs are currently available, but their uses are limited by a number of factors, such as low potency, poor solubility, microbial resistance, and drug toxicity. Therefore, the search for new and more effective antimicrobial agents and the development of alternative therapies, such as PACT, are necessary. Crude hexane and ethanol extracts of A. maritima were produced. The prepared extracts presented absorption at 650-700 nm. For bioassays, 50 mu L of culture medium, 50 mu L of extract (25 mg/mL) or control, and 5 mu L of a suspension of the microorganism to be tested (C. dubliniensis ATCC 778157 or ATCC 777, 10(7) CFU/mL) were placed in a sterile 96-well microtiter plate (well cross section = 0.38 cm(2)). The contents of each well were irradiated with a 685-nm diode laser with an output power of 35 mW, which was distributed through the well cross section yielding an energy dosage of 28 J/cm(2). In each assay (n = 6), one plate was subjected to irradiation, and one was not. For each active sample, the number of colony-forming units per milliliter (CFU/mL) was obtained, and data were analyzed by the Tukey test. The chemical compositions of the extracts were determined by chromatographic and spectroscopic techniques. The results suggest inhibition of the growth of C dubliniensis when irradiated with a diode laser in the presence of hexane and ethanol extracts from A. maritima as photosensitizers. Laser irradiation alone or crude extracts at 25 mg/mL did not significantly reduce the number of CFU/mL. Steroids, triterpenes, and flavonoids were identified in the analyzed extracts. In conclusion, the photoactivation of crude hexane and ethanol extracts of A. maritima by red laser radiation at 685 nm promoted an antimicrobial effect, showing that these natural products can be used as photosensitizers in PACT. (C) 2010 Elsevier B.V. All rights reserved.
dc.description99
dc.description1
dc.description15
dc.description20
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAEPEX-UNICAMP
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Sa
dc.publisherLausanne
dc.publisherSuíça
dc.relationJournal Of Photochemistry And Photobiology B-biology
dc.relationJ. Photochem. Photobiol. B-Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectPDT
dc.subjectPACT
dc.subjectPhotosensitizer
dc.subjectCandida-dubliniensis
dc.subjectFlavonoids
dc.subjectPhotofrin
dc.subjectAlbicans
dc.subjectTherapy
dc.titleExtracts from Alternanthera maritima as natural photosensitizers in photodynamic antimicrobial chemotherapy (PACT)
dc.typeArtículos de revistas


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