Stimulation of potato tuber respiration by cold stress is associated with an increased capacity of both plant uncoupling mitochondrial protein (PUMP) and alternative oxidase

dc.creatorCalegario, FF
dc.creatorCosso, RG
dc.creatorFagian, MM
dc.creatorAlmeida, FV
dc.creatorJardim, WF
dc.creatorJezek, P
dc.creatorArruda, P
dc.creatorVercesi, AE
dc.date2003
dc.dateJUN
dc.date2014-11-17T07:40:29Z
dc.date2015-11-26T17:11:40Z
dc.date2014-11-17T07:40:29Z
dc.date2015-11-26T17:11:40Z
dc.date.accessioned2018-03-29T00:00:08Z
dc.date.available2018-03-29T00:00:08Z
dc.identifierJournal Of Bioenergetics And Biomembranes. Kluwer Academic/plenum Publ, v. 35, n. 3, n. 211, n. 220, 2003.
dc.identifier0145-479X
dc.identifierWOS:000183994500003
dc.identifier10.1023/A:1024655514113
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79889
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79889
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79889
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281150
dc.descriptionThe CO2 evolution of intact potato tubers (Solanum tuberosum, L., var. 'Bintje') was analyzed during a 10-day period of their warm (25+/-2degreesC) or cold (5+/-1degreesC) storage, to evaluate cold-stress effects on expression and activities of plant uncoupling mitochondrial protein (PUMP) and alternative oxidase (AOX). CO2 evolution rates were analyzed at 20degreesC, to reflect their possible capacities. The 20degreesC CO2 production declined from 13 to 8 mg kg(-1) h(-1) after 2 days of warm storage and then (after 3 to 7 days) decreased from 8 to 6.5 mg kg(-1) h(-1). In contrast, 20degreesC CO2 evolution did not change after the first day of cold storage, increased up to 14.5 mg kg(-1) h(-1) after 2 days, and decreased to about 12 mg kg(-1) h(-1) after 3 to 7 days of cold storage. Cold storage increased PUMP expression as detected by Western blots and led to elevated capacities of both PUMP (44%) and CN-resistant AOX (10 times), but not the cytochrome pathway. Since we found that cold storage led to about the same mitochondrial respiration of 40 nmol O-2 min(-1) mg(-1) attributable to each of the respective proteins, we conclude that both AOX and PUMP equally contribute to adaptation of potato tubers to cold.
dc.description35
dc.description3
dc.description211
dc.description220
dc.languageen
dc.publisherKluwer Academic/plenum Publ
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectwhole potato tuber respiration
dc.subjectpotato tuber mitochondria
dc.subjectplant uncoupling mitochondrial protein
dc.subjectalternative oxidase
dc.subjectcold stress
dc.subjectTomato Fruit Mitochondria
dc.subjectImmunochemical Affinity
dc.subjectArabidopsis-thaliana
dc.subjectCyanide-resistant
dc.subjectSeed-tubers
dc.subjectWinter Rye
dc.subject310 Kd
dc.subjectActivation
dc.subjectGeneration
dc.subjectMechanism
dc.titleStimulation of potato tuber respiration by cold stress is associated with an increased capacity of both plant uncoupling mitochondrial protein (PUMP) and alternative oxidase
dc.typeArtículos de revistas


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