Fungal laccase: copper induction, semi-purification, immobilization, phenolic effluent treatment and electrochemical measurement

dc.creatorCordi, L
dc.creatorMinussi, RC
dc.creatorFreire, RS
dc.creatorDuran, N
dc.date2007
dc.dateMAY 16
dc.date2014-11-13T21:07:03Z
dc.date2015-11-26T17:11:26Z
dc.date2014-11-13T21:07:03Z
dc.date2015-11-26T17:11:26Z
dc.date.accessioned2018-03-28T23:59:56Z
dc.date.available2018-03-28T23:59:56Z
dc.identifierAfrican Journal Of Biotechnology. Academic Journals, v. 6, n. 10, n. 1255, n. 1259, 2007.
dc.identifier1684-5315
dc.identifierWOS:000248685000018
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67699
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/67699
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67699
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281100
dc.descriptionThe laccase activity induced by copper ions in Trametes versicolor by two inducers was studied and the aims were to show that it is possible to obtain a high laccase activity in almost 12 of the enzymes product using a basic culture medium but different copper concentration. Laccase was inducted by 2,5-xylidine and copper sulfate. Semi-purification of the crude laccase was carried out through precipitation and the column separation with NaCl gradient. In order to apply in an effluent treatment, laccase was immobilized on different vitroceramics supports, pyrolytic graphite and also on a carbon fiber electrode as biosensor. The maximum laccase activity was 40,774.0 U L-1 at the 12(th) day. The best support for immobilization was pyrolytic graphite ( glutaraldehyde treated-94% efficiency). Total phenol removal in Kraft E1 effluent was 19% for immobilized pyrolytic graphite with the mediator present. The biosensor prepared with this material showed a good linear response to catechol. The optimization of laccase activity induction through the combination of 2,5-xylidine and cooper sulfate was obtained and led to its use in environmental remediation.
dc.description6
dc.description10
dc.description1255
dc.description1259
dc.languageen
dc.publisherAcademic Journals
dc.publisherNairobi
dc.relationAfrican Journal Of Biotechnology
dc.relationAfr. J. Biotechnol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectTrametes versicolor
dc.subjectlaccase induction
dc.subjectsemi-purification
dc.subjectimmobilization
dc.subjecteffluent treatment
dc.subjectTrametes-versicolor
dc.subjectPotential Applications
dc.subjectWaste-water
dc.subjectDecolorization
dc.subjectDegradation
dc.subjectPeroxidases
dc.subjectImprovement
dc.subjectBioreactor
dc.subjectExpression
dc.subjectManganese
dc.titleFungal laccase: copper induction, semi-purification, immobilization, phenolic effluent treatment and electrochemical measurement
dc.typeArtículos de revistas


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