Traveling-Wave Ion Mobility Mass Spectrometry Analysis of Isomeric Modified Peptides Arising from Chemical Cross-Linking

dc.creatorSantos, LFA
dc.creatorIglesias, AH
dc.creatorPilau, EJ
dc.creatorGomes, AF
dc.creatorGozzo, FC
dc.date2010
dc.dateDEC
dc.date2014-11-13T19:41:55Z
dc.date2015-11-26T17:11:17Z
dc.date2014-11-13T19:41:55Z
dc.date2015-11-26T17:11:17Z
dc.date.accessioned2018-03-28T23:59:49Z
dc.date.available2018-03-28T23:59:49Z
dc.identifierJournal Of The American Society For Mass Spectrometry. Springer, v. 21, n. 12, n. 2062, n. 2069, 2010.
dc.identifier1044-0305
dc.identifierWOS:000284985200013
dc.identifier10.1016/j.jasms.2010.08.017
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77318
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77318
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77318
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281067
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionTraveling-wave ion mobility (TWIM) coupled to mass spectrometry (MS) has emerged as a powerful tool for structural and conformational analysis of proteins and peptides, allowing the analysis of isomeric peptides (or proteins) with the same sequence but modified at different residues This work demonstrates the use of the novel TWIM-MS technique to separate isomeric peptide ions derived from chemical cross-linking experiments, which enables the acquisition of distinct product ion spectra for each isomer, clearly indicating modification on different sites Experiments were performed with four synthetic peptides, for which variable degrees of mobility separation were achieved In cases of partially overlapping mobility arrival time distributions (ATDs), extracting the ATDs of fragment ions belonging to each individual isomer allowed their separation into two distinct ATDs Accumulation over regions from the specific ATDs generates the product ion spectrum of each isomer, or a spectrum highly enriched in their fragments The population of both modified peptide isomers was correlated with the intrinsic reactivities of different Lys residues from reactions conducted at different pH conditions (J Am Soc Mass Spectrom 2010, 21, 2062-2069) (C) 2010 Published by Elsevier Inc on behalf of American Society for Mass Spectrometry
dc.description21
dc.description12
dc.description2062
dc.description2069
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [FAPESP 2004/14,846-0, FINEP 01 07 0290 00]
dc.descriptionFAPESP [FAPESP 08/57,805-2, CNPq 573672/2008-3]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of The American Society For Mass Spectrometry
dc.relationJ. Am. Soc. Mass Spectrom.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectGas-phase
dc.subjectPhosphorylated Peptides
dc.subjectAspiration Condenser
dc.subjectProtein Complexes
dc.subjectLysine Residues
dc.subjectLinked Peptides
dc.subjectDrift Cell
dc.subjectIdentification
dc.subjectArchitecture
dc.subjectPropionate)
dc.titleTraveling-Wave Ion Mobility Mass Spectrometry Analysis of Isomeric Modified Peptides Arising from Chemical Cross-Linking
dc.typeArtículos de revistas


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