Discovery of immune-related genes expressed in hemocytes of the tarantula spider Acanthoscurria gomesiana

dc.creatorLorenzini, DM
dc.creatorSilva, PID
dc.creatorSoares, MB
dc.creatorArruda, P
dc.creatorSetubal, J
dc.creatorDaffre, S
dc.date2006
dc.date2014-11-20T01:13:53Z
dc.date2015-11-26T17:11:05Z
dc.date2014-11-20T01:13:53Z
dc.date2015-11-26T17:11:05Z
dc.date.accessioned2018-03-28T23:59:36Z
dc.date.available2018-03-28T23:59:36Z
dc.identifierDevelopmental And Comparative Immunology. Pergamon-elsevier Science Ltd, v. 30, n. 6, n. 545, n. 556, 2006.
dc.identifier0145-305X
dc.identifierWOS:000237270000003
dc.identifier10.1016/j.dci.2005.09.001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79699
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79699
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79699
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281016
dc.descriptionThe present study reports the identification of immune related transcripts from hemocytes of the spider Acanthoscurria gomesiana by high throughput sequencing of expressed sequence tags (ESTs). To generate ESTs from hemocytes, two cDNA libraries were prepared: one by directional cloning (primary) and the other by the normalization of the first (normalized). A total of 7584 clones were sequenced and the identical ESTs were clustered, resulting in 3723 assembled sequences (AS). At least 20% of these sequences are putative novel genes. The automatic functional annotation of AS based on Gene Ontology revealed several abundant transcripts related to the following functional classes: hemocyanin, lectin, and structural constituents of ribosome and cytoskeleton. From this annotation, 73 transcripts possibly involved in immune response were also identified, suggesting the existence of several molecular processes not previously described for spiders, such as: pathogen recognition, coagulation, complement activation, cell adhesion and intracellular signaling pathway for the activation of cellular defenses. (c) 2005 Elsevier Ltd. All rights reserved.
dc.description30
dc.description6
dc.description545
dc.description556
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationDevelopmental And Comparative Immunology
dc.relationDev. Comp. Immunol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecthemocytes
dc.subjectinnate immunity
dc.subjectexpressed sequence tags (ESTs)
dc.subjecthemocyanin
dc.subjectcoagulation
dc.subjectlectins
dc.subjectserine-proteases
dc.subjectantimicrobial peptides
dc.subjectCrab Antimicrobial Peptides
dc.subjectSequence Tag Analysis
dc.subjectHorseshoe-crab
dc.subjectInnate Immunity
dc.subjectAnopheles-gambiae
dc.subjectPhenoloxidase Activity
dc.subjectDrosophila Toll
dc.subjectHost-defense
dc.subjectInfection
dc.subjectInvertebrate
dc.titleDiscovery of immune-related genes expressed in hemocytes of the tarantula spider Acanthoscurria gomesiana
dc.typeArtículos de revistas


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