The effects of nicotine and cotinine on Porphyromonas gingivalis colonisation of epithelial cells

dc.creatorCogo, K
dc.creatorCalvi, BM
dc.creatorMariano, FS
dc.creatorFranco, GCN
dc.creatorGoncalves, RB
dc.creatorGroppo, FC
dc.date2009
dc.dateNOV
dc.date2014-11-20T00:51:19Z
dc.date2015-11-26T17:10:43Z
dc.date2014-11-20T00:51:19Z
dc.date2015-11-26T17:10:43Z
dc.date.accessioned2018-03-28T23:59:16Z
dc.date.available2018-03-28T23:59:16Z
dc.identifierArchives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 54, n. 11, n. 1061, n. 1067, 2009.
dc.identifier0003-9969
dc.identifierWOS:000271439500012
dc.identifier10.1016/j.archoralbio.2009.08.001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73813
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73813
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73813
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280931
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSmoking is a risk factor for development of periodontitis. Porphyromonas gingivalis is an important colonizer of the subgingival crevice and is a major pathogenic agent in the initiation and progression of severe forms of periodontal disease. However, the effect of major cigarette's derivatives on P. gingivalis is poorly understood. The purpose of this study was to determine the influence of nicotine and cotinine on bacterial colonisation to epithelial cells. KB cells monolayers and P. gingivalis ATCC 33277 were exposed to 0.1, 10 and 100 mu g/ml, of nicotine and cotinine concentrations. The epithelial cells were incubated for 24 h while P. gingivalis was exposed to these substances until reach early logarithmic phase. After the incubation period, P. gingivalis ability to colonize KB cells was assayed. The number of cell-associated/invasive bacteria was assessed by counting the colony-forming unities. 100 mu g/ml. cotinine significantly increased P. gingivalis association and invasion of epithelial cells, when the bacteria was exposed to this substance (p < 0.05; ANOVA - Tukey test). No other condition or drug altered the bacteria colonisation ability (p > 0.05). These data indicated that cotinine may interfere with P. gingivalis ability to associate and invade the epithelial cells. Further studies are needed to investigate whether oral cells might be more susceptible to be colonized by P. gingivalis in smokers. (C) 2009 Elsevier Ltd. All rights reserved.
dc.description54
dc.description11
dc.description1061
dc.description1067
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [06/60619-0]
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationArchives Of Oral Biology
dc.relationArch. Oral Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBacterial association
dc.subjectBacterial invasion
dc.subjectCells
dc.subjectEpithelial
dc.subjectPorphyromonas gingivalis
dc.subjectNicotine
dc.subjectCotinine
dc.subjectCigarette-smoking
dc.subjectPeriodontal-disease
dc.subjectBacterial Adherence
dc.subjectIn-vitro
dc.subjectFibroblasts
dc.subjectAttachment
dc.subjectInvasion
dc.subjectSmokers
dc.subjectInfection
dc.subjectPathogens
dc.titleThe effects of nicotine and cotinine on Porphyromonas gingivalis colonisation of epithelial cells
dc.typeArtículos de revistas


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