The effect of IFN-gamma and TNF-alpha on the eosinophilic differentiation and NADPH oxidase activation of human HL-60 clone 15 cells

dc.creatorLopez, JA
dc.creatorNewburger, PE
dc.creatorCondino-Neto, A
dc.date2003
dc.dateDEC
dc.date2014-11-13T17:34:14Z
dc.date2015-11-26T17:10:43Z
dc.date2014-11-13T17:34:14Z
dc.date2015-11-26T17:10:43Z
dc.date.accessioned2018-03-28T23:59:16Z
dc.date.available2018-03-28T23:59:16Z
dc.identifierJournal Of Interferon And Cytokine Research. Mary Ann Liebert Inc Publ, v. 23, n. 12, n. 737, n. 744, 2003.
dc.identifier1079-9907
dc.identifierWOS:000187925700017
dc.identifier10.1089/107999003772084851
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/75054
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/75054
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/75054
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280929
dc.descriptionThe aim of this study was to investigate the effect of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) on NADPH oxidase activity and gp91-phox gene expression in HL-60 clone 15 cells as they differentiate along the eosinophilic lineage. The results were compared to the eosoniphilic inducers interleukin-5 (IL-5) and butyric acid. IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml) or IL-5 (200 pM) caused a significant increase in the expression of the eosinophil peroxidase (EPO) and the major basic protein (MBP) genes. Similar results were observed when the cells were cultured with 0.5 mM butyric acid for 5 days. IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml) also caused a significant increase in superoxide release by HL-60 clone 15 cells after 2 days compared with control or with butyric acid-induced cells. After 5 days, these cytokines and butyric acid induced an even stronger release of superoxide. HL-60 clone 15 cells cultured with IFN-gamma and TNF-alpha for 2 days showed a significant increase in gp91-phox gene expression. We conclude that IFN-gamma and TNF-alpha are sufficient to induce the differentiation of HL-60 clone 15 cells to the eosinophilic lineage and to upregulate gp91-phox gene expression and activity of the NADPH oxidase system.
dc.description23
dc.description12
dc.description737
dc.description744
dc.languageen
dc.publisherMary Ann Liebert Inc Publ
dc.publisherLarchmont
dc.publisherEUA
dc.relationJournal Of Interferon And Cytokine Research
dc.relationJ. Interferon Cytokine Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectChronic Granulomatous-disease
dc.subjectRecombinant Interferon Gamma
dc.subjectInterleukin-5 Messenger-rna
dc.subjectColony-stimulating Factor
dc.subjectChain Gene-expression
dc.subjectTumor-necrosis-factor
dc.subjectCytochrome-b Heavy
dc.subjectIn-vitro
dc.subjectSuperoxide-production
dc.subjectBlood Eosinophils
dc.titleThe effect of IFN-gamma and TNF-alpha on the eosinophilic differentiation and NADPH oxidase activation of human HL-60 clone 15 cells
dc.typeArtículos de revistas


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