DNA ploidy analysis in salivary gland tumours by image cytometry

dc.creatorVargas, PA
dc.creatorTorres-Rendon, A
dc.creatorSpeight, PM
dc.date2007
dc.dateJUL
dc.date2014-11-13T16:39:10Z
dc.date2015-11-26T17:10:30Z
dc.date2014-11-13T16:39:10Z
dc.date2015-11-26T17:10:30Z
dc.date.accessioned2018-03-28T23:59:04Z
dc.date.available2018-03-28T23:59:04Z
dc.identifierJournal Of Oral Pathology & Medicine. Blackwell Publishing, v. 36, n. 6, n. 371, n. 376, 2007.
dc.identifier0904-2512
dc.identifierWOS:000247135500009
dc.identifier10.1111/j.1600-0714.2007.00551.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62403
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/62403
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62403
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280875
dc.descriptionAim: To determine whether DNA ploidy by image cytometry is a good diagnostic tool to distinguish benign and malignant salivary gland tumours. Methods: A total of 62 salivary gland tumours were studied. Cases were histologically diagnosed [haematoxylin and eosin (H&E)]. According to the World Health Organization (WHO) classification, there were 14 mucoepidermoid carcinomas (MEC), 11 adenoid cystic carcinomas (ACC), 10 pleomorphic adenomas (PA), 10 carcinoma ex PA (CEPA), 9 acinic cell carcinomas (ACCa), 3 polymorphous low-grade adenocarcinomas (PLGA), 2 papillary cystadenocarcinomas (PC), 1 myoepithelial carcinoma (MC), 1 undifferentiated carcinoma (UC) and 1 mucinous adenocarcinoma (MA). Paraffin sections (40 mu m) were micro-dissected to isolate tumour areas; cell nuclei were extracted and Feulgen-stained cytospin monolayers were analysed using a DNA image cytometry system. For each case, DNA index (DI) was calculated relative to internal controls (lymphocytes; DI = 1.0). Cases were categorized as diploid or aneuploid and the proportion of cells over 5c was also calculated. Results: Fifty-three of 62 salivary gland tumours were uniformly diploid. Only nine cases were aneuploid: five CEPA, one low-grade MEC, one PC, one UC and one MA. Conclusions: The vast majority of salivary gland tumours were diploid. High-grade malignancies may be aneuploid, and ploidy may be useful to identify malignant change in atypical PA. Further, larger studies are needed to confirm our results and to further evaluate the usefulness of the technique in high-grade lesions.
dc.description36
dc.description6
dc.description371
dc.description376
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Oral Pathology & Medicine
dc.relationJ. Oral Pathol. Med.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectaneuploidy
dc.subjectcarcinoma ex pleomorphic adenoma
dc.subjectDNA image cytometry
dc.subjectdiagnosis
dc.subjectploidy
dc.subjectsalivary gland tumours
dc.subjectFlow-cytometry
dc.subjectDuct Carcinoma
dc.subjectParotid-gland
dc.subjectMucoepidermoid Carcinoma
dc.subjectPrognostic-significance
dc.subjectPleomorphic Adenomas
dc.subjectCell-carcinoma
dc.subjectMalignancy
dc.subjectAneuploidy
dc.subjectDiagnosis
dc.titleDNA ploidy analysis in salivary gland tumours by image cytometry
dc.typeArtículos de revistas


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