Metabolism of aromatic hydrocarbons by the filamentous fungus Cyclothyrium sp.

dc.creatorda Silva, M
dc.creatorEsposito, E
dc.creatorJoanna, DMC
dc.creatorCanhos, VP
dc.creatorCerniglia, CE
dc.date2004
dc.dateNOV
dc.date2014-11-20T00:34:42Z
dc.date2015-11-26T17:10:28Z
dc.date2014-11-20T00:34:42Z
dc.date2015-11-26T17:10:28Z
dc.date.accessioned2018-03-28T23:59:02Z
dc.date.available2018-03-28T23:59:02Z
dc.identifierChemosphere. Pergamon-elsevier Science Ltd, v. 57, n. 8, n. 943, n. 952, 2004.
dc.identifier0045-6535
dc.identifierWOS:000224879400020
dc.identifier10.1016/j.chemosphere.2004.07.051
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56560
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/56560
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56560
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280869
dc.descriptionThe metabolism of biphenyl, naphthalene, anthracene, phenanthrene, pyrene and benzo[a]pyrene by Cyclothyrium sp. CBS 109850, a coelomycete isolated for the first time in Brazil from industrially polluted estuarine sediment, was studied. The metabolites were extracted and separated by high performance liquid chromatography (HPLC) and characterized by UV spectral analyses and mass, and proton nuclear magnetic resonance (H-1 NMR) spectrometry. Cyclothyrium sp. transformed biphenyl to 4-hydroxybiphenyl and anthracene to anthracene trans-1,2-dihydrodiol. This isolate metabolized 90% of [9-C-14] phenanthrene, producing phenanthrene trans-9,10-dihydrodiol as a major metabolite, phenanthrene trans-3,4-dihydrodiol, 1-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, and a novel metabolite, 2-hydroxy-7-methoxyphenanthrene. Circular dichroism spectra analyses indicated that the major enantiomers of phenanthrene trans-9, 10-dihydrodiol, phenanthrene trans-3,4-dihydrodiol and pyrene trans-4,5-dihydrodiol, a pyrene metabolite produced previously by Cyclothyrium sp. CBS 109850, were predominantly in the (R,R) configuration, revealing a high stereoselectivity for initial monooxygenation and enzymatic hydration of phenanthrene and pyrene by Cyclothyrium sp. CBS109850. The results also show a high regioselectivity since the K-regions of phenanthrene and pyrene were the major sites of metabolism. (C) 2004 Elsevier Ltd. All rights reserved.
dc.description57
dc.description8
dc.description943
dc.description952
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationChemosphere
dc.relationChemosphere
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectfungi
dc.subjectpollutants
dc.subjectaromatic hydrocarbons
dc.subjectbiotransformation
dc.subjectcoelomycetes
dc.subjectRat-liver Microsomes
dc.subjectSp Strain Pyr-1
dc.subjectCunninghamella-elegans
dc.subjectPleurotus-ostreatus
dc.subjectStereoselective Metabolism
dc.subjectEstuarine Sediments
dc.subjectPhenanthrene
dc.subjectPyrene
dc.subjectOxidation
dc.subjectDegradation
dc.titleMetabolism of aromatic hydrocarbons by the filamentous fungus Cyclothyrium sp.
dc.typeArtículos de revistas


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