Preparation and characterization of diacetylene polymerized liposomes for detection of autoantibodies

dc.creatorCabral, ECM
dc.creatorHennies, PT
dc.creatorCorreia, CRD
dc.creatorZollner, RL
dc.creatorSantana, MHA
dc.date2003
dc.date2014-11-13T15:04:29Z
dc.date2015-11-26T17:10:13Z
dc.date2014-11-13T15:04:29Z
dc.date2015-11-26T17:10:13Z
dc.date.accessioned2018-03-28T23:58:49Z
dc.date.available2018-03-28T23:58:49Z
dc.identifierJournal Of Liposome Research. Marcel Dekker Inc, v. 13, n. 41732, n. 199, n. 211, 2003.
dc.identifier0898-2104
dc.identifierWOS:000186991200003
dc.identifier10.1081/LPR-120026387
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70094
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70094
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70094
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280813
dc.descriptionAffinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 63 5 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases.
dc.description13
dc.description41732
dc.description199
dc.description211
dc.languageen
dc.publisherMarcel Dekker Inc
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Liposome Research
dc.relationJ. Liposome Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectpolymerized liposomes
dc.subjectimmunoassays
dc.subjectcardiolipin
dc.subjectanti-cardiolipin antibodies
dc.subjectSystemic Lupus-erythematosus
dc.subjectAnticardiolipin Antibodies
dc.subjectPolydiacetylene Vesicles
dc.subjectColorimetric Detection
dc.subjectImmunoassays
dc.subjectColor
dc.titlePreparation and characterization of diacetylene polymerized liposomes for detection of autoantibodies
dc.typeArtículos de revistas


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