Genotyping of human cytomegalovirus using non-radioactive single-strand conformation polymorphism (SSCP) analysis

dc.creatorde Albuquerque, DM
dc.creatorCosta, SCB
dc.date2003
dc.date39965
dc.date2014-11-13T14:58:25Z
dc.date2015-11-26T17:10:13Z
dc.date2014-11-13T14:58:25Z
dc.date2015-11-26T17:10:13Z
dc.date.accessioned2018-03-28T23:58:48Z
dc.date.available2018-03-28T23:58:48Z
dc.identifierJournal Of Virological Methods. Elsevier Science Bv, v. 110, n. 1, n. 25, n. 28, 2003.
dc.identifier0166-0934
dc.identifierWOS:000183336200004
dc.identifier10.1016/S0166-0934(03)00094-6
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67969
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/67969
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67969
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280810
dc.descriptionGenetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant sequence or a polymorphism in a known gene. SSCP analysis has the advantage over RFLP analysis on detection of DNA polymorphisms and point mutations at a variety of positions in DNA fragments. However, the original SSCP protocols using the incorporation of radioactive label and polyacrylamide gel electrophoresis for detection are labour intensive and time-consuming. A simplified SSCP protocol is described to identify HCMV strains and the gB genotype, allowing the detection of sequence variations not residing in the endonuclease recognition sites. (C) 2003 Elsevier Science B.V. All rights reserved.
dc.description110
dc.description1
dc.description25
dc.description28
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Virological Methods
dc.relationJ. Virol. Methods
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecthuman cytomegalovirus
dc.subjectglycoprotein B
dc.subjectstrains/variants
dc.subjectPCR-restriction fragment length polymorphism
dc.subjectPCR-single-strand conformation polymorphism
dc.subjectPolymerase Chain-reaction
dc.subjectMarrow Transplant Recipients
dc.subjectGlycoprotein-b Genotypes
dc.subjectInterstrain Variation
dc.subjectSequence Variation
dc.subjectReaction Products
dc.subjectDna-sequences
dc.subjectFrequency
dc.subjectVariants
dc.subjectStrains
dc.titleGenotyping of human cytomegalovirus using non-radioactive single-strand conformation polymorphism (SSCP) analysis
dc.typeArtículos de revistas


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