Transcription of succinate dehydrogenase subunit 4 (sdh4) gene in potato: detection of extensive RNA editing and co-transcription with cytochrome oxidase subunit III (cox3) gene

dc.creatorSiqueira, SF
dc.creatorDias, SMG
dc.creatorHardouin, P
dc.creatorPereira, FRS
dc.creatorLejeune, B
dc.creatorde Souza, AP
dc.date2002
dc.dateJUL
dc.date2014-11-13T14:10:04Z
dc.date2015-11-26T17:09:51Z
dc.date2014-11-13T14:10:04Z
dc.date2015-11-26T17:09:51Z
dc.date.accessioned2018-03-28T23:58:29Z
dc.date.available2018-03-28T23:58:29Z
dc.identifierCurrent Genetics. Springer-verlag, v. 41, n. 4, n. 282, n. 289, 2002.
dc.identifier0172-8083
dc.identifierWOS:000177738100012
dc.identifier10.1007/s00294-002-0294-y
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77498
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77498
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77498
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280725
dc.descriptionThe potato (Solanum tuberosum L.) mitochondrial cox3/sdh4 gene cluster was previously identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In this work, this potato gene cluster was cloned, sequenced and its expression was evaluated. The gene sequence and gene locus organization were found to be similar to the corresponding gene cluster in other dicot species, where known. Northern hybridizations with potato and cauliflower (Brassica oleraceae) mtRNA and RT-PCR analyses using potato mtRNA indicated that cox3 and sdh4 are co-transcribed in both species, generating a complex transcription pattern, where several transcripts from 1.1 kb to 4.4 kb are found. The potato transcript from this cluster displayed 14 and 13 RNA-editing sites, in the cox3 and sdh4 genes respectively, which changed the codon identity to amino acids and created a sdh4 partially edited stop codon. Forty-three cDNA clones were analyzed for editing process and revealed different partial-editing with no apparent sequential processing in the sdh4 gene.
dc.description41
dc.description4
dc.description282
dc.description289
dc.languageen
dc.publisherSpringer-verlag
dc.publisherNew York
dc.publisherEUA
dc.relationCurrent Genetics
dc.relationCurr. Genet.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectmitochondrial DNA
dc.subjectcytochrome oxidase subunit III
dc.subjectsuccinate dehydrogenase subunit 4
dc.subjectRNA editing
dc.subjectpotato
dc.subjectPlant Mitochondrial Genomes
dc.subjectNucleotide-sequence
dc.subjectComplex-ii
dc.subjectWheat
dc.subjectOrganization
dc.subjectNucleus
dc.subjectDna
dc.subjectEvolution
dc.subjectIdentification
dc.subjectCoxiii
dc.titleTranscription of succinate dehydrogenase subunit 4 (sdh4) gene in potato: detection of extensive RNA editing and co-transcription with cytochrome oxidase subunit III (cox3) gene
dc.typeArtículos de revistas


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