Effect of phosphate concentration on the production of dextransucrase by Leuconostoc mesenteroides NRRL B512F

dc.creatorRodrigues, S
dc.creatorLona, LMF
dc.creatorFranco, TT
dc.date2003
dc.dateNOV
dc.date2014-11-13T11:46:05Z
dc.date2015-11-26T17:09:21Z
dc.date2014-11-13T11:46:05Z
dc.date2015-11-26T17:09:21Z
dc.date.accessioned2018-03-28T23:58:00Z
dc.date.available2018-03-28T23:58:00Z
dc.identifierBioprocess And Biosystems Engineering. Springer-verlag, v. 26, n. 1, n. 57, n. 62, 2003.
dc.identifier1615-7591
dc.identifierWOS:000186589200007
dc.identifier10.1007/s00449-003-0330-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64401
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64401
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64401
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280604
dc.descriptionLeuconostoc mesenteroides NRRL B512F is the main strain used in industrial fermentations to produce dextransucrase and dextran. This process has been studied since the Second World War, when it was used as blood plasma expander. A study about the effect of phosphate concentration on cell propagation in a semicontinuous shake-flask culture is described in this work. Dextransucrase is obtained by fermentation of the Leuconostoc mesenteroides NRRL B512F in the presence of sucrose as substrate, a nitrogen source (corn liquor or yeast extract) and minerals. Phosphate is currently used in order to buffer the culture medium. Cell propagation can be done through a repeated batch culture, where dilution in a fresh medium is made with relatively short periods. The standard medium for dextransucrase production is prepared using 0.1 M of K2HPO4. In this work the level of phosphate was increased to 0.3 M, and an increase on biomass and on the enzyme activity was found when phosphate enriched medium was used. Higher phosphate buffer concentration was also able to keep the pH values above 5.0 during the entire process, avoiding enzyme denaturation.
dc.description26
dc.description1
dc.description57
dc.description62
dc.languageen
dc.publisherSpringer-verlag
dc.publisherNew York
dc.publisherEUA
dc.relationBioprocess And Biosystems Engineering
dc.relationBioprocess. Biosyst. Eng.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectdextransucrase
dc.subjectshake-flask fermentation
dc.subjectLeuconostoc mesenteroides NRRL B512F
dc.subjectNrrl B-512f
dc.titleEffect of phosphate concentration on the production of dextransucrase by Leuconostoc mesenteroides NRRL B512F
dc.typeArtículos de revistas


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