Effect of high intensity aerobic exercise and mesterolone on remodeling of Achilles tendon of C57BL/6 transgenic mice

dc.creatorFontana, K
dc.creatorAlmeida, FM
dc.creatorTomiosso, TC
dc.creatorPimentel, ER
dc.creatorHofling, MADC
dc.date2010
dc.dateFEB
dc.date2014-11-13T11:53:41Z
dc.date2015-11-26T17:09:19Z
dc.date2014-11-13T11:53:41Z
dc.date2015-11-26T17:09:19Z
dc.date.accessioned2018-03-28T23:57:58Z
dc.date.available2018-03-28T23:57:58Z
dc.identifierCell And Tissue Research. Springer, v. 339, n. 2, n. 411, n. 420, 2010.
dc.identifier0302-766X
dc.identifierWOS:000274331000010
dc.identifier10.1007/s00441-009-0894-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64405
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64405
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64405
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280594
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe effect of mesterolone and intensive treadmill training (6 weeks, 5 days/week, means: 15.82 m/min and 45.8 min/day) in Achilles tendon remodeling was evaluated. Sedentary mice treated with mesterolone (Sed-M) or vehicle (Sed-C, placebo/control) and corresponding exercised (Ex-M and Ex-C) were examined. SDS-polyacrylamide gel electrophoresis was used for determining collagen bands and hydroxyproline concentration. Collagen fibril diameter, the area and number of fibrils contained in an area probe, and the ultrastructure of fibroblasts (tenocytes) were determined. The presence of collagen was notable in the tendons of all groups. Collagen alpha(1/)alpha(2) bands in Sed-M, Ex-C, and Ex-M were higher than in Sed-C, as shown by hydroxyproline content, but collagen beta-chain appeared only in Ex-C. Noticeable bands of non-collagenous proteins were found in Sed-M and Ex-M. The number of fibrils in the area probe increased markedly in Sed-M and Ex-C (12-fold), but their diameter and area were unchanged compared with Sed-C. In Ex-M, the fibril number decreased by three-fold to 3.5-fold compared with Sed-M and Ex-C, whereas diameter and area increased. Sed-C tenocytes appeared quiescent, whereas those in the other groups seemed to be engaged in protein synthesis. The density of tenocytes was smaller in Sed-C than in Ex-C, Sed-M, and Ex-M. Thus, mechanical stimuli and mesterolone alter the morphology of tenocytes and the composition of the tendon, probably through fibrillogenesis and/or increased intermolecular cross-links. The ergogenic effect is evidenced by the activation of collagenous and non-collagenous protein synthesis and the increase in the diameter and area of collagen fibrils. This study might be relevant to clinical sports medicine.
dc.description339
dc.description2
dc.description411
dc.description420
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [04/13767-9]
dc.descriptionCNPq [522131/95-6]
dc.descriptionFAPESP [04/13768-5]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationCell And Tissue Research
dc.relationCell Tissue Res.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectAnabolic androgenic steroid
dc.subjectFibril collagen diameter
dc.subjectFibril collagen area
dc.subjectHydroxyproline
dc.subjectNon-collagenous proteins
dc.subjectMouse (C57BL/6)
dc.subjectAnabolic-androgenic Steroids
dc.subjectCollagen-fibril Populations
dc.subjectSkeletal-muscle
dc.subjectRat Tendon
dc.subjectMechanical-properties
dc.subjectExtracellular-matrix
dc.subjectReceptor
dc.subjectExpression
dc.subjectAtherosclerosis
dc.subjectUltrastructure
dc.titleEffect of high intensity aerobic exercise and mesterolone on remodeling of Achilles tendon of C57BL/6 transgenic mice
dc.typeArtículos de revistas


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