Bile and liver metallothionein behavior in copper-exposed fish

dc.creatorHauser-Davis, RA
dc.creatorBastos, FF
dc.creatorTuton, B
dc.creatorRocha, RC
dc.creatorSt' Pierre, T
dc.creatorZiolli, RL
dc.creatorArruda, MAZ
dc.date2014
dc.date2014-07-30T13:51:55Z
dc.date2015-11-26T17:09:16Z
dc.date2014-07-30T13:51:55Z
dc.date2015-11-26T17:09:16Z
dc.date.accessioned2018-03-28T23:57:55Z
dc.date.available2018-03-28T23:57:55Z
dc.identifierJournal Of Trace Elements In Medicine And Biology. Elsevier Gmbh, Urban & Fischer Verlag, v. 28, n. 1, n. 70, n. 74, 2014.
dc.identifier0946-672X
dc.identifierWOS:000330911600015
dc.identifier10.1016/j.jtemb.2013.09.003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55468
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55468
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280584
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe present study analyzed metallothionein (MT) excretion from liver to bile in Nile Tilapia (Oreochromis niloticus) exposed to sub-lethal copper concentrations (2 mg L-1) in a laboratory setting. MTs in liver and bile were quantified by spectrophotometry after thermal incubation and MT metal-binding profiles were characterized by size exclusion high performance liquid chromatography coupled to ICP-MS (SEC-HPLC-ICP-MS). Results show that liver MT is present in approximately 250-fold higher concentrations than bile MT in non-exposed fish. Differences between the MT profiles from the control and exposed group were observed for both matrices, indicating differential metal-binding behavior when comparing liver and bile MT. This is novel data regarding intra-organ MT comparisons, since differences between organs are usually present only with regard to quantification, not metal-binding behavior. Bile MT showed statistically significant differences between the control and exposed group, while the same did not occur with liver MT. This indicates that MTs synthesized in the liver accumulate more slowly than MTs excreted from liver to bile, since the same fish presented significantly higher MT levels in liver when compared to bile. We postulate that bile, although excreted in the intestine and partially reabsorbed by the same returning to the liver, may also release MT-bound metals more rapidly and efficiently, which may indicate an efficient detoxification route. Thus, we propose that the analysis of bile MTs to observe recent metal exposure may be more adequate than the analysis of liver MTs, since organism responses to metals are more quickly observed in bile, although further studies are necessary. (C) 2013 Elsevier GmbH. All rights reserved.
dc.description28
dc.description1
dc.description70
dc.description74
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.publisherJena
dc.publisherAlemanha
dc.relationJournal Of Trace Elements In Medicine And Biology
dc.relationJ. Trace Elem. Med. Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectMetallothionein
dc.subjectBile
dc.subjectLiver
dc.subjectCu contamination
dc.subjectFish
dc.subjectIcp-ms
dc.subjectMetal Speciation
dc.subjectErod Activity
dc.subjectQuantification
dc.subjectBiomarkers
dc.subjectExcretion
dc.subjectProteins
dc.subjectEstuary
dc.subjectFlesus
dc.subjectTrout
dc.titleBile and liver metallothionein behavior in copper-exposed fish
dc.typeArtículos de revistas


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