Beta cell coupling and connexin expression change during the functional maturation of rat pancreatic islets

dc.creatorCarvalho, CPF
dc.creatorBarbosa, HCL
dc.creatorBritan, A
dc.creatorSantos-Silva, JCR
dc.creatorBoschero, AC
dc.creatorMeda, P
dc.creatorCollares-Buzato, CB
dc.date2010
dc.dateJUL
dc.date2014-11-19T23:08:29Z
dc.date2015-11-26T17:09:01Z
dc.date2014-11-19T23:08:29Z
dc.date2015-11-26T17:09:01Z
dc.date.accessioned2018-03-28T23:57:41Z
dc.date.available2018-03-28T23:57:41Z
dc.identifierDiabetologia. Springer, v. 53, n. 7, n. 1428, n. 1437, 2010.
dc.identifier0012-186X
dc.identifierWOS:000278118700021
dc.identifier10.1007/s00125-010-1726-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55422
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55422
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55422
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280522
dc.descriptionCell-cell coupling mediated by gap junctions formed from connexin (CX) contributes to the control of insulin secretion in the endocrine pancreas. We investigated the cellular production and localisation of CX36 and CX43, and gap junction-mediated beta cell coupling in pancreatic islets from rats of different ages, displaying different degrees of maturation of insulin secretion. The presence and distribution of islet connexins were assessed by immunoblotting and immunofluorescence. The expression of connexin genes was evaluated by RT-PCR and quantitative real-time PCR. The ultrastructure of gap junctions and the function of connexin channels were assessed by freeze-fracture electron microscopy and tracer microinjection, respectively. Young and adult beta cells, which respond to glucose, expressed significantly higher levels of Cx36 (also known as Gjd2) than fetal and newborn beta cells, which respond poorly to the sugar. Accordingly, adult beta cells also showed a significantly higher membrane density of gap junctions and greater intercellular exchange of ethidium bromide than newborn beta cells. Cx43 (also known as Gja1) was not expressed by beta cells, but was located in various cell types at the periphery of fetal and newborn islets. These findings show that the pattern of connexins, gap junction membrane density and coupling changes in islets during the functional maturation of beta cells.
dc.description53
dc.description7
dc.description1428
dc.description1437
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationDiabetologia
dc.relationDiabetologia
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectBeta cells
dc.subjectCell-cell coupling
dc.subjectConnexins
dc.subjectGene expression
dc.subjectDevelopment
dc.subjectGap junction
dc.subjectInsulin secretion
dc.subjectMaturation
dc.subjectPancreatic islet
dc.subjectInsulin-producing Cells
dc.subjectGap-junction Channels
dc.subjectIn-vivo Maturation
dc.subjectB-cells
dc.subjectCa2+ Oscillations
dc.subjectStem-cells
dc.subjectSecretion
dc.subjectGlucose
dc.subjectFetal
dc.subjectCommunication
dc.titleBeta cell coupling and connexin expression change during the functional maturation of rat pancreatic islets
dc.typeArtículos de revistas


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