Beta-lactamic Resistance Profiles in Porphyromonas, Prevotella, and Parvimonas Species Isolated from Acute Endodontic Infections

dc.creatorMontagner, F
dc.creatorJacinto, RC
dc.creatorSignoretti, FGC
dc.creatorde Mattos, VS
dc.creatorGrecca, FS
dc.creatorGomes, BPFDA
dc.date2014
dc.dateMAR
dc.date2014-07-30T13:51:51Z
dc.date2015-11-26T17:08:57Z
dc.date2014-07-30T13:51:51Z
dc.date2015-11-26T17:08:57Z
dc.date.accessioned2018-03-28T23:57:37Z
dc.date.available2018-03-28T23:57:37Z
dc.identifierJournal Of Endodontics. Elsevier Science Inc, v. 40, n. 3, n. 339, n. 344, 2014.
dc.identifier0099-2399
dc.identifier1878-3554
dc.identifierWOS:000333722100003
dc.identifier10.1016/j.joen.2013.10.037
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55435
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55435
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280507
dc.descriptionIntroduction: Susceptibility to beta-lactamic agents has changed among anaerobic isolates from acute endodontic infections. The aim of the present study was to determine the prevalence of the cfxAlcfxA2 gene in Prevotella spp., Porphyromonas spp., and Parviomonas micra strains and show its phenotypic expression. Methods: Root canal samples from teeth with acute endodontic infections were collected and Porphyromonas, Prevotella, and Parvimonas micra strains were isolated and microbiologically identified with conventional culture techniques. The susceptibility of the isolates was determined by the minimum inhibitory concentration of benzylpenicillin, amoxicillin, and amoxicillin + clavulanate using the E-test method (AB BIODISK, Solna, Sweden). The presence of the cfxAlcfxA2 gene was determined through primer-specific polymerase chain reaction. The nitrocefin test was used to determine the expression of the lactamase enzyme. Results: Prevotella disiens, Prevotella oralis, Porphyromonas gin givalis, and P micra strains were susceptible to benzylpenicillin, amoxicillin, and amoxicillin + clavulanate. The cfxA/cfxA2 gene was detected in 2 of 29 isolates (6.9%). Simultaneous detection of the cfxAlcfxA2 gene and lactamase production was observed for 1 Prevotella buccalis strain. The gene was in 1 P micra strain but was not expressed. Three strains were positive for lactamase production, but the cfxAlcfxA2 gene was not detected through polymerase chain reaction. Conclusions: There is a low prevalence of the cfxAl cfxA2 gene and its expression in Porphyromonas spp., Prevotella spp., and P. micra strains isolated from acute endodontic infections. Genetic and phenotypic screening must be performed simultaneously to best describe additional mechanisms involved in lactamic resistance for strict anaerobes.
dc.description40
dc.description3
dc.description339
dc.description344
dc.descriptionFederal University of Rio Grande do Sul [2012-7178]
dc.descriptionVanessa S Matos (PROBIC_FAPERGS/UFRGS)
dc.descriptionFederal University of Rio Grande do Sul [2012-7178]
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Endodontics
dc.relationJ. Endod.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAnaerobic bacteria
dc.subjectantimicrobial agents
dc.subjectE-test
dc.subjectlactamases
dc.subjectnitrocefin
dc.subjectpolymerase chain reaction
dc.subjectsusceptibility
dc.subjectOrofacial Odontogenic Infections
dc.subjectAntimicrobial Susceptibility
dc.subjectAntibiotic-resistance
dc.subjectEnterococcus-faecalis
dc.subjectPeriodontal Pockets
dc.subjectRoot Canals
dc.subjectBacteria
dc.subjectAbscesses
dc.subjectPathogens
dc.subjectGenes
dc.titleBeta-lactamic Resistance Profiles in Porphyromonas, Prevotella, and Parvimonas Species Isolated from Acute Endodontic Infections
dc.typeArtículos de revistas


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