Iron-responsive genes of Phanerochaete chrysosporium isolated by differential display reverse transcription polymerase chain reaction

dc.creatorAssmann, EM
dc.creatorOttoboni, LMM
dc.creatorFerraz, A
dc.creatorRodriguez, J
dc.creatorde Mello, MP
dc.date2003
dc.dateSEP
dc.date2014-11-19T23:03:04Z
dc.date2015-11-26T17:08:54Z
dc.date2014-11-19T23:03:04Z
dc.date2015-11-26T17:08:54Z
dc.date.accessioned2018-03-28T23:57:34Z
dc.date.available2018-03-28T23:57:34Z
dc.identifierEnvironmental Microbiology. Wiley-blackwell, v. 5, n. 9, n. 777, n. 786, 2003.
dc.identifier1462-2912
dc.identifierWOS:000184744200007
dc.identifier10.1046/j.1462-2920.2003.00475.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60733
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60733
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60733
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280492
dc.descriptionWhite-rot fungus Phanerochaete chrysosporium , a ligninolytic basidiomycete, was studied to identify iron-responsive genes. Using the differential display reverse transcription PCR technique (DDRT-PCR), a total of 97 differentially expressed cDNA fragments were identified by comparing band intensities among fingerprints obtained from mycelia cultivated in iron-deficient and iron-replete media. Transcripts induced under iron-starvation exhibited homologies to: a modular polyketide synthase, a TonB protein, a probable transmembrane protein, a putative ABC transporter permease and a HSP70-related heat-shock protein. Modular polyketide synthase and TonB proteins are normally expressed under iron-starvation and are known to be involved in biosynthesis and transport of siderophores respectively. Also, a deduced protein with 96% similarity to a precursor of the well-known P. chrysosporium lignin peroxidase was identified under iron-deficiency. Two DDRT-PCR products confirmed their iron-induced expression. One was homologue to the CNOT3, which is a global regulator of RNA polymerase II transcription and has been implicated in multiple roles in the control of mRNA metabolism. The other was similar to the Schizosaccharomyces pombe putative proteasome maturation factor upm1. In conclusion, the majority of iron-responsive P. chrysosporium transcripts isolated in the DDRT-PCR encode proteins involved in iron acquisition, especially members of biosynthesis and transport of iron chelators.
dc.description5
dc.description9
dc.description777
dc.description786
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationEnvironmental Microbiology
dc.relationEnviron. Microbiol.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectSaccharomyces-cerevisiae
dc.subjectSiderophore Biosynthesis
dc.subjectUstilago-maydis
dc.subjectGloeophyllum-trabeum
dc.subjectTransport-system
dc.subjectRegulated Genes
dc.subjectFungi
dc.subjectWood
dc.subjectAcquisition
dc.subjectPathways
dc.titleIron-responsive genes of Phanerochaete chrysosporium isolated by differential display reverse transcription polymerase chain reaction
dc.typeArtículos de revistas


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