Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish

dc.creatorHauser-Davis, RA
dc.creatorBastos, FF
dc.creatorDantas, RF
dc.creatorTobar, SAL
dc.creatorNeto, JDB
dc.creatorBastos, VLFD
dc.creatorZiolli, RL
dc.creatorArruda, MAZ
dc.date2014
dc.dateMAY
dc.date2014-07-30T13:51:47Z
dc.date2015-11-26T17:08:49Z
dc.date2014-07-30T13:51:47Z
dc.date2015-11-26T17:08:49Z
dc.date.accessioned2018-03-28T23:57:30Z
dc.date.available2018-03-28T23:57:30Z
dc.identifierEcotoxicology And Environmental Safety. Academic Press Inc Elsevier Science, v. 103, n. 24, n. 28, 2014.
dc.identifier0147-6513
dc.identifier1090-2414
dc.identifierWOS:000334395400004
dc.identifier10.1016/j.ecoenv.2014.01.015
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55392
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55392
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280473
dc.descriptionThe pacu (Piaractus mesopotamicus) is a hypoxia-tolerant neotropical fish species. There is little or no information in this species regarding biochemical adaptations to waters with different oxygen concentrations, such as the production of reactive oxygen species and antioxidant scavengers, which might be of interest in the study of antioxidant defense mechanisms. Metallothioneins (MT) have been widely applied as biomarkers for metal exposure in fish liver, and, recently, in bile. These metalloproteins, however, have also been reported as free radical scavengers, although studies in this regard are scarce in fish. In this context, normoxic and hypoxic controlled experiments were conducted with pacu specimens and MT levels were quantified in both liver and bile. Reduced glutathione (GSH) indicative of oxidative stress, and thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, were also determined in liver. The results demonstrate that hypoxic fish present significantly lower metallothionein levels in liver and bile and lower reduced glutathione levels in liver, whereas lipid peroxidation was not significantly different between hypoxic and normoxic fish. The results of the present study seem to suggest that metallothioneins may actively participate in redox regulation in hypoxic fish in both bile and liver. MT levels in these organs may be temporarily suppressed, supporting the notion that down-regulation of oxidant scavengers during the oxidative burst is important in defense signaling in these adapted organisms. (C) 2014 Elsevier Inc. All rights reserved.
dc.description103
dc.description24
dc.description28
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationEcotoxicology And Environmental Safety
dc.relationEcotox. Environ. Safe.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectMetallothionein
dc.subjectGSH
dc.subjectTBARS
dc.subjectFish bile and liver
dc.subjectHypoxia
dc.subjectPacu Piaractus-mesopotamicus
dc.subjectAntioxidant Defenses
dc.subjectOxidative Stress
dc.subjectPantanal Wetland
dc.subjectInducible Factor
dc.subjectTolerant Fish
dc.subjectGlutathione
dc.subjectTissue
dc.subjectOxygen
dc.subjectLiver
dc.titleBehaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish
dc.typeArtículos de revistas


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