Quercetin diminishes the binding of hemoglobin to the red blood cell membrane

dc.creatorCesquini, M
dc.creatorTenor, AC
dc.creatorTorsoni, MA
dc.creatorStoppa, GR
dc.creatorPereira, AL
dc.creatorOgo, SH
dc.date2001
dc.dateSPR
dc.date2014-11-19T20:42:27Z
dc.date2015-11-26T17:08:40Z
dc.date2014-11-19T20:42:27Z
dc.date2015-11-26T17:08:40Z
dc.date.accessioned2018-03-28T23:57:18Z
dc.date.available2018-03-28T23:57:18Z
dc.identifierJournal Of Anti-aging Medicine. Mary Ann Liebert Inc, v. 4, n. 1, n. 55, n. 63, 2001.
dc.identifier1094-5458
dc.identifierWOS:000168650600008
dc.identifier10.1089/109454501750225695
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58762
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58762
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58762
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280426
dc.descriptionHemoglobin (Hb) oxidation leads to the formation of hemichrome, which binds to the membrane and causes red blood cell removal by the reticuloendothelial system. In the present investigation, the effect of flavonoids on Hb oxidation and their binding to red blood cell (RBC) membranes were studied using tert-butyl hydroperoxide (tert-BOOH) to promote oxidative stress. The intrinsic antioxidant activity of RBC was able to prevent the binding of Hb to the membrane at tert-BOOH concentrations up to 0.4 mM. At higher concentrations, a brown pellet was observed and represented the appearance of membrane-bound oxidized Hb. Oxidations performed in membrane-free Hb solutions with an identical oxidative system showed less Hb oxidation. These observations suggest that erythrocyte membrane lipid peroxidation enhances the oxidative damage of Hb, increasing its binding to membranes. Quercetin partially protected Hb against oxidation by tert-BOOH and reduced the levels of the membrane bound hemichrome. Lipid peroxidation was also significantly suppressed by quercetin. Rutin and morin had little effect in preventing Hb binding to RBC membranes, indicating the importance of structure in the antioxidant properties of flavonoids. In the absence of oxidant, the peroxidation of erythrocyte membrane and isotonic hemolysis were protected by quercetin. These results suggest that quercetin displays a beneficial role on aging of RBC.
dc.description4
dc.description1
dc.description55
dc.description63
dc.languageen
dc.publisherMary Ann Liebert Inc
dc.publisherNew Rochelle
dc.publisherEUA
dc.relationJournal Of Anti-aging Medicine
dc.relationJ. Anti-Aging Med.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectErythrocyte-membrane
dc.subjectLipid-peroxidation
dc.subjectHydrogen-peroxide
dc.subjectOxidative Damage
dc.subjectRate Constants
dc.subjectFlavonoids
dc.subjectAntioxidant
dc.subjectBand-3
dc.subjectSpectrin
dc.subjectRadicals
dc.titleQuercetin diminishes the binding of hemoglobin to the red blood cell membrane
dc.typeArtículos de revistas


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