Morphological modifications of knee articular cartilage in bullfrogs (Lithobates catesbeianus) (Anura: Ranidae) during postmetamorphic maturation

dc.creatorHebling, A
dc.creatorEsquisatto, MAM
dc.creatorAro, AA
dc.creatorGomes, L
dc.date2014
dc.dateJUN
dc.date2014-08-01T18:41:49Z
dc.date2015-11-26T17:08:14Z
dc.date2014-08-01T18:41:49Z
dc.date2015-11-26T17:08:14Z
dc.date.accessioned2018-03-28T23:56:51Z
dc.date.available2018-03-28T23:56:51Z
dc.identifierZoomorphology. Springer, v. 133, n. 2, n. 245, n. 256, 2014.
dc.identifier0720-213X
dc.identifier1432-234X
dc.identifierWOS:000336051700012
dc.identifier10.1007/s00435-014-0218-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/82217
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/82217
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280310
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThe organization of knee articular cartilage of the bullfrog (Lithobates catesbeianus) differs in relation to morphofunctional adaptation in many aspects from similar structures in mammals. Thus, we investigated the structural organization and distribution of the extracellular matrix components in three articular cartilage regions in the distal epiphysis of the femur and proximal epiphysis of the tibia in male bullfrogs at 7, 540 and 1,080 days after metamorphosis. Cartilage thickness and cell density decreased in all regions with age. The basophilia differed among cartilage sites during aging. Calcium deposits were detected in growth cartilage of the femur and tibia in older animals. Immunohistochemical staining for chondroitin-6-sulfate was positive in the pericellular and territorial matrix in all samples. Positive immunostaining for type I collagen was observed in the superficial layer at all ages and in ossification centers of older animals. Reactivity to type II collagen was intense and was found throughout the stroma at all ages. Ultrastructural analysis of the epiphyseal region, in young animals, showed that the cytoplasm of chondrocytes was rich in rough endoplasmic reticulum, Golgi complex and mitochondria. In old animals, were observed a reduction in the size and number of mitochondria, disintegration of rough endoplasmic reticulum, and vacuolization of the Golgi complex. The bullfrog articular cartilage presented structural and organizational changes during aging which may contribute to the functional cartilage deterioration in old animals.
dc.description133
dc.description2
dc.description245
dc.description256
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundacao Herminio Ometto
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationZoomorphology
dc.relationZoomorphology
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectAging
dc.subjectArticular cartilage
dc.subjectExtracellular matrix
dc.subjectBullfrog
dc.subjectJoints
dc.subjectAge-related-changes
dc.subjectRana-catesbeiana
dc.subjectEpiphyseal Cartilage
dc.subjectFibrillar Collagens
dc.subjectTemporal Patterns
dc.subjectGrowth Cartilage
dc.subjectLong Bones
dc.subjectChondrocyte
dc.subjectOsteoarthritis
dc.subjectUltrastructure
dc.titleMorphological modifications of knee articular cartilage in bullfrogs (Lithobates catesbeianus) (Anura: Ranidae) during postmetamorphic maturation
dc.typeArtículos de revistas


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