Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate-co-hydroxyvalerate) as surfaces for Vero cell culture

dc.creatorSantos, AR
dc.creatorFerreira, BMP
dc.creatorDuek, EAR
dc.creatorDolder, H
dc.creatorWada, MLF
dc.date2005
dc.dateNOV
dc.date2014-11-19T16:55:44Z
dc.date2015-11-26T17:07:19Z
dc.date2014-11-19T16:55:44Z
dc.date2015-11-26T17:07:19Z
dc.date.accessioned2018-03-28T23:55:51Z
dc.date.available2018-03-28T23:55:51Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 38, n. 11, n. 1623, n. 1632, 2005.
dc.identifier0100-879X
dc.identifierWOS:000232915100009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73257
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73257
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73257
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280094
dc.descriptionVero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37 degrees C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, and 0/100). The present study evaluated morphology of cells 40/60, grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis, and stained with Toluidine blue, pH 4.0, and Xylidine ponceau, pH 2.5. Our results showed that cell adhesion was better when 60/40 and 50/50 blends were used although cells were able to grow and proliferate on all blends tested. When using PLLA/PHBV (50150) slightly flattened cells were observed on porous and smooth areas. PLLA/PHBV (40/60) blends presented flattened cells on smooth areas. PLLA/PHBV (0/100), which presented no pores, also supported spreading cells interconnected by thin filaments. Histological sections showed that cells grew as a confluent monolayer on different substrates. Cytochemical analysis showed basophilic cells, indicating a large amount of RNA and proteins. Hence, we detected changes in cell morphology induced by alterations in blend proportions. This suggests that the cells changed their differentiation pattern when on various PLLA/PHBV blend surfaces.
dc.description38
dc.description11
dc.description1623
dc.description1632
dc.languageen
dc.publisherAssoc Bras Divulg Cientifica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Medical And Biological Research
dc.relationBrazilian J. Med. Biol. Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectpoly(L-lactic acid)
dc.subjectpoly(hydroxybutyrate-co-hydroxyvlerate)
dc.subjectblends
dc.subjectbiomaterials
dc.subjectcell culture
dc.subjectVero cells
dc.subjectExtracellular-matrix
dc.subjectArticular-cartilage
dc.subjectIn-vitro
dc.subjectMorphology
dc.subjectGrowth
dc.subjectDifferentiation
dc.subjectBiomaterials
dc.subjectProliferation
dc.subjectRegeneration
dc.subjectChondrocytes
dc.titleUse of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate-co-hydroxyvalerate) as surfaces for Vero cell culture
dc.typeArtículos de revistas


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