Inflammatory oedema induced by phospholipases A(2) isolated from Crotalus durissus sp in the rat dorsal skin: a role for mast cells and sensory C-fibers

dc.creatorCamara, PRS
dc.creatorEsquisatto, LCM
dc.creatorCamargo, EA
dc.creatorTeresa, M
dc.creatorRibela, MTCP
dc.creatorToyama, MH
dc.creatorMarangoni, S
dc.creatorDe Nucci, G
dc.creatorAntunes, E
dc.date2003
dc.dateJUN
dc.date2014-11-19T15:26:37Z
dc.date2015-11-26T17:07:02Z
dc.date2014-11-19T15:26:37Z
dc.date2015-11-26T17:07:02Z
dc.date.accessioned2018-03-28T23:55:30Z
dc.date.available2018-03-28T23:55:30Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 41, n. 7, n. 823, n. 829, 2003.
dc.identifier0041-0101
dc.identifierWOS:000183685300011
dc.identifier10.1016/S0041-0101(03)00037-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60160
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60160
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60160
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280013
dc.descriptionThe ability of the phospholipases A(2) (PLA(2)s) from Crotalus durissus cascavella, Crotalus durissus collilineatus and Crotalus durissus terrificus venoms and crotapotin to increase the vascular permeability in the rat skin as well as the contribution of both mast cells and sensory C-fibers have been investigated in this study. Vascular permeability was measured as the plasma extravascular accumulation at skin sites of intravenously injected I-125-human serum albumin. Intradermal injection of crotalic PLA(2)s (0.05-0.5 mug/site) in the rat skin resulted in dose-dependent increase in plasma extravascular whereas crotapotin (1 mug/site) failed to affect this response. Co-injection of crotapotin (1 mug/site) did not modify the increased vascular permeability induced by the PLA(2)s (0.05-0.5 mug/site). Previous treatment (30 min) of the animals with cyproheptadine (2 mg/kg, i.p.) markedly reduced PLA(2) (0.5 mug/site)-induced oedema. In rats treated neonatally with capsaicin to deplete neuropeptides, the plasma extravasation induced by all PLA(2)s (0.5 mug/site) was also significantly reduced. Similarly, the tachykinin NK1 receptor antagonist SR140333 (1 nmol/site) significantly reduced the PLA(2)-induced oedema. In addition, the combination of SR140333 with cyproheptadine further reduced the increased plasma extravasation by PLA(2) from C. d. cascavella venom, but not by PLA(2) from C d. terrificus and C. d. collilineatus venoms. Our results suggest that increase in skin vascular permeability by crotalic PLA(2)s is mediated by activation of sensory C-fibers culminating in the release of substance P, as well as by activation of mast cells which in turn release amines such as histamine and serotonin. (C) 2003 Elsevier Science Ltd. All rights reserved.
dc.description41
dc.description7
dc.description823
dc.description829
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectneurogenic inflammation
dc.subjectmast cells
dc.subjectphospholipase A(2)
dc.subjectvenoms
dc.subjectsensory fibers
dc.subjectSubstance-p
dc.subjectEnzymatic Characterization
dc.subjectVenom Phospholipase-a2
dc.subjectBiological-activities
dc.subjectSnake-venom
dc.subjectGuinea-pig
dc.subjectPaw Edema
dc.subjectCrotoxin
dc.subjectAntagonist
dc.subjectPeptides
dc.titleInflammatory oedema induced by phospholipases A(2) isolated from Crotalus durissus sp in the rat dorsal skin: a role for mast cells and sensory C-fibers
dc.typeArtículos de revistas


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